1x1 image pixel for data collection
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix
Product Image
Gibco™

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS)Read more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
A14132011 mL
A14132025 mL
Catalog number A1413201
Price (USD)
82.50
Each
Add to cart
Quantity:
1 mL
Price (USD)
82.50
Each
Add to cart
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. The major components of Geltrex™ matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycans. It is ideal for the promotion and maintenance of differentiated phenotypes in a variety of cell cultures including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC). Geltrex™ matrix offers, on average, a 2-fold reduction in several growth factors. As a result, analysis of specific growth factor effects in applications requiring basement membrane extract can be carried out in a controlled and reproducible format.

Key Features of Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix:

• Free of lactose dehydrogenase elevating virus (LDEV) making it ideal for all types of cell culture and mouse in vivo applications
• Consistent protein concentration lot-to-lot helps reduce the need to screen lots
• Protocols for various applications, including tube formation assays, are available

Gain Reliability and Consistency in Your hESC Studies
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus free. Additionally, our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, averaging 15 mg/ml so you can have confidence in every purchase.

Investigate Angiogenesis with Validated Gibco™ Protocols
There are many applications for Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, each requiring different thicknesses and concentrations. In general, a protein concentration of >5 mg/ml is used for differentiation studies of primary cells. Geltrex™ LDEV-Free reduced growth factor basement membrane matrix has been used to develop tube formation assay protocols using Gibco™ primary cells and specialty media. See this and other angiogenesis assay protocols
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeVariety of cultured cells, including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC)
Concentration12 to 18 mg/mL
Endotoxin LevelLow
FormatTube(s)
Product LineGibco™
Product TypeBasement Membrane Matrix
Purity or Quality GradeCell Culture Grade
Quantity1 mL
Shelf Life36 months
Shipping ConditionDry Ice
SpeciesMouse
ClassificationReduced Growth Factor
Culture TypeAdherent Cell Culture
FormFrozen
Serum LevelSerum-free
SterilitySterile
Without AdditivesNo Phenol Red
Unit SizeEach
Contents & Storage
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matric comes as a sterile protein solution in 1 mL vials.

Store at -20 to -80°C.

Geltrex™ Matrix is stable for 18 months from date of manufacture when properly stored.

Frequently asked questions (FAQs)

What growth factors are reduced in the Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413202)?

Although the standard basement membrane extract (BME) contains growth factors such as EGF, IGF‐1, PDGF and TGF‐beta, the reduced growth factor basement membrane extract product (Geltrex matrix) contains below detectable levels of EGF, PDGF and NGF. It still has some TGF‐beta (about 2 ng/mL) and 3 times less IGF‐1 on average than regular BME, hence the designation "reduced". Neither standard nor reduced BME contain NGF or FGF‐2.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center

What volume do you recommend for thin coating 35 mm dishes with Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413201, A1413202) or Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302)?

We recommend thin (non-gelling) coating 35 mm dishes with 1.5 mL of Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413201, A1413202) or Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302) prediluted 1/100 in DMEM/F-12 medium (1% final concentration).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I refreeze and reuse Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix after having used it in an experimental setup?

Yes, you can refreeze and reuse Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix. However, you should avoid multiple freeze/thaw cycles. hence, we would recommend the following:

  1. Thaw Geltrex Matrix in a refrigerator overnight at 2-8 degrees C.
  2. Mix Geltrex Matrix solution by slowly pipetting up and down. Be careful not to introduce air bubbles.
  3. If not using up the complete solution, prepare multiple aliquots, either of the original solution or the diluted one, and freeze.


Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I characterize human embryonic stem ( ES) cells?

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).

How are human embryonic stem (ES) cells derived?

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link