HEPES, 99%
Thermo Scientific Chemicals

HEPES, 99%

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50 g
10 g
250 g
Catalog number A14777.18
also known as A14777-18
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52.65
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58.10 
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Quantity:
50 g
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Price (USD)/ Each
52.65
Online exclusive
58.10 
Save 5.45 (9%)
Add to cart
HEPES, 99%
Catalog numberA14777.18
Price (USD)/ Each
52.65
Online exclusive
58.10 
Save 5.45 (9%)
-
Add to cart
Chemical Identifiers
CAS7365-45-9
IUPAC Name2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
Molecular FormulaC8H18N2O4S
InChI KeyJKMHFZQWWAIEOD-UHFFFAOYSA-N
SMILESOCCN1CCN(CCS(O)(=O)=O)CC1
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SpecificationsSpecification SheetSpecification Sheet
FormCrystals or powder or crystalline powder
Assay (Non-aqueous acid-base Titration)≥98.5 to ≤101.5% (non-U.S. specification)
Assay from Supplier's CofA≥98.5% (U.S. specification)
Identification (FTIR)Conforms (non-U.S. specification)
Appearance (Color)White
HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.

Solubility
Soluble in water.

Notes
Incompatible with strong oxidizing agents.  
RUO – Research Use Only

General References:

  1. Maus, S.; Breeman, W.; Buchholz, H. G.; de Blois, E.; Schreckenberger, M. Simple and reliable chromatographic technique for the determination of HEPES in Ga-68-labeled pharmaceuticals. J. Nucl. Med. 2015, 56, 1168-1168.
  2. Taha, M.; Khoiroh, I.; Lee, M. J. Phase behavior and molecular dynamics simulation studies of new aqueous two-phase separation systems induced by HEPES buffer. J. Phys. Chem. B 2013, 117, (2), 563-582.