Neurite Outgrowth Staining Kit
Neurite Outgrowth Staining Kit
Invitrogen™

Neurite Outgrowth Staining Kit

The Molecular Probes™ Neurite Outgrowth Staining Kit allows for quick and simple measurement of neurite outgrowth and cell viability inRead more
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Catalog NumberQuantity
A150011000 assays
Catalog number A15001
Price (USD)
478.00
Each
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Quantity:
1000 assays
Recurring order eligible. Learn more »
Price (USD)
478.00
Each
Add to cart
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The Molecular Probes™ Neurite Outgrowth Staining Kit allows for quick and simple measurement of neurite outgrowth and cell viability in the same sample. It includes three fluorescent dyes: a cell viability indicator, a cell membrane stain, and a background suppression reagent. Please note that the dyes included in the kit are not specific to neurons, and will stain any animal cells. This kit is not suitable for distinguishing neurons from other cell types in a mixed population.

  • Stain cells faster than with immunocytochemistry (in as little as 15 minutes)
  • Measure neurite outgrowth and cell health in the same sample
  • Image either live or fixed cells
  • Capture data with a standard fluorescence microscope, microplate reader, or HCS instrument

Rapid Staining Results
The Neurite Outgrowth Staining Kit saves time and steps relative to immunostaining. A typical workflow using this kit takes 15–30 minutes and involves only two media-exchange steps prior to taking a measurement. In contrast, immunostaining involves multiple block, wash, and antibody incubation steps that require several hours to complete.

Multiplexed Readouts
The Neurite Outgrowth Staining Kit distinguishes between compound effects on cell viability and neurite outgrowth by providing a dual color readout.

Flexible Protocol
Depending on the cell type of interest, a fixed-cell staining approach may be preferable to live-cell staining. Both protocols are provided in the product manual for maximum flexibility.

Instrument Compatibility
The fluorescence signals generated with the Neurite Outgrowth Staining Kit can be measured with a fluorescence microplate reader, microscope, or HCS instrument for compatibility with your existing workflow. Likewise, the probes used in this kit are compatible with standard FITC (green) and TRITC (orange-red) emission filter sets.

What You Get:
Easily scalable to any culture plate, dish, or slide format, the Neurite Outgrowth Staining Kit comes with reagents sufficient to run 1,000 96-well assays, and includes:

  • 100 μL of Cell Viability Indicator (1000X)
  • 100 μL of Cell Membrane Stain (1000X)
  • 2.5 mL of Background Suppression Dye (100X)

For Research Use Only. Not for use in diagnostic procedures.

Specifications
ColorGreen, Orange
Quantity1000 assays
Recommended Storage100 μL 1000X Cell Viability Indicator
100 μL 1000X Cell Membrane Stain
2.5 mL 100X Background Suppression Dye

Each component stored at -20°C.
TypeNeurite Outgrowth Staining Kit
Unit SizeEach
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
2945662Certificate of AnalysisApr 09, 2024A15001
2901460Certificate of AnalysisApr 09, 2024A15001.18, A15001, A15001.22, A15001.36
2857316Certificate of AnalysisJan 25, 2024A15001
2834649Certificate of AnalysisOct 31, 2023A15001
2805447Certificate of AnalysisOct 12, 2023A15001
5 results displayed, search above for a specific certificate

Safety Data Sheets

Frequently asked questions (FAQs)

No, the viability dye and membrane stain can stain any cell type. Other Neurite Outgrowth Staining Kit FAQs can be found here (https://www.thermofisher.com/content/dam/LifeTech/migration/files/cell-tissue-analysis/pdfs.par.90941.file.dat/neurite-outgrowth-staining-kit-faqs.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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