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Catalog Number | Quantity |
---|---|
A15001 | 1000 assays |
The Molecular Probes™ Neurite Outgrowth Staining Kit allows for quick and simple measurement of neurite outgrowth and cell viability in the same sample. It includes three fluorescent dyes: a cell viability indicator, a cell membrane stain, and a background suppression reagent. Please note that the dyes included in the kit are not specific to neurons, and will stain any animal cells. This kit is not suitable for distinguishing neurons from other cell types in a mixed population.
Rapid Staining Results
The Neurite Outgrowth Staining Kit saves time and steps relative to immunostaining. A typical workflow using this kit takes 15–30 minutes and involves only two media-exchange steps prior to taking a measurement. In contrast, immunostaining involves multiple block, wash, and antibody incubation steps that require several hours to complete.
Multiplexed Readouts
The Neurite Outgrowth Staining Kit distinguishes between compound effects on cell viability and neurite outgrowth by providing a dual color readout.
Flexible Protocol
Depending on the cell type of interest, a fixed-cell staining approach may be preferable to live-cell staining. Both protocols are provided in the product manual for maximum flexibility.
Instrument Compatibility
The fluorescence signals generated with the Neurite Outgrowth Staining Kit can be measured with a fluorescence microplate reader, microscope, or HCS instrument for compatibility with your existing workflow. Likewise, the probes used in this kit are compatible with standard FITC (green) and TRITC (orange-red) emission filter sets.
What You Get:
Easily scalable to any culture plate, dish, or slide format, the Neurite Outgrowth Staining Kit comes with reagents sufficient to run 1,000 96-well assays, and includes:
For Research Use Only. Not for use in diagnostic procedures.
No, the viability dye and membrane stain can stain any cell type. Other Neurite Outgrowth Staining Kit FAQs can be found here (https://www.thermofisher.com/content/dam/LifeTech/migration/files/cell-tissue-analysis/pdfs.par.90941.file.dat/neurite-outgrowth-staining-kit-faqs.pdf).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
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