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TaqMan™ hPSC Scorecard™ Panel, Fast 96-well
TaqMan™ hPSC Scorecard™ Panel, Fast 96-well
Applied Biosystems™

TaqMan™ hPSC Scorecard™ Panel, Fast 96-well

The Applied Biosystems™ TaqMan™ hPSC Scorecard™ Panel 2 x 96w FAST enables verification of pluripotency and determination of lineage biasRead more
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Catalog NumberQuantity
A158762 x 96-well plates
Catalog number A15876
Price (USD)
1,012.00
Each
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Quantity:
2 x 96-well plates
Price (USD)
1,012.00
Each
Add to cart
The Applied Biosystems™ TaqMan™ hPSC Scorecard™ Panel 2 x 96w FAST enables verification of pluripotency and determination of lineage bias for both ES and iPS cell lines. Each of the two 96-well plates contains 94 predefined TaqMan™ Gene Expression assays (including endogenous controls) dried-down in the wells. Master mix is not included with this panel. For a kit that combines the panel with master mix, see TaqMan™ hPSC Scorecard™ Kit 2 x 96w FAST. This product is also available in a 384-well format.

• Assess an entire gene signature in one simple experiment
• Compare results to a reference standard with the accompanying hPSC Scorecard™ Analysis Software
• Identify lines with trilineage differentiation potential
• Evaluate inductive differentiation specified to one germ layer
• Access validated content for increased confidence in your results

Assess an Entire Gene Signature in One Experiment
The TaqMan™ hPSC Scorecard™ Panel helps save time by offering pre-plated assays in a stable and convenient dried-down format. Simply add TaqMan™ Master Mix to your cDNA of interest and transfer to the 96-well plate with a multichannel pipette.

Compare Results to a Reference Standard
The TaqMan™ hPSC Scorecard™ Panel includes access to proprietary cloud-based analysis software that allows you to view and export graphs and tables of results, as well as generate reports. The software is compatible with seven Applied Biosystems™ qRT-PCR systems and is available at no additional charge.

Identify Lines with Trilineage Differentiation Potential
Include randomly differentiated embryoid body (EB) samples (differentiated for at least 7 days using standard EB methods) in your analysis and determine whether your lines are biased toward one or more of the three germ layers (endoderm, mesoderm, ectoderm).

Evaluate Inductive Differentiation Specified to One Germ Layer
The panel includes over twenty markers for each of the three germ layers that have been shown to respond as expected to directed differentiation in monolayer culture. Quickly evaluate time course data, culture conditions and media formulations for your germ layer of interest with this one panel.

Access Validated Content
The panel contents are based on published work (Bock et al., Cell 144, 439–452, 2011) and were validated against multiple human ES and iPS lines.

What You Get
The TaqMan™ hPSC Scorecard™ Panel 2 x 96w FAST includes:

• Two 96-well plates of 94 TaqMan™ Gene Expression assays
• Two optical plate covers
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-Probe Detection
For Use With (Equipment)QuantStudio™ 12k Flex, StepOne™, Fast Mode, ViiA™ 7 System
FormDried Down
Includes2 x 96-well plate
Product LineTaqMan™
Quantity2 x 96-well plates
Reaction SpeedFast
Research CategoryStem Cell Research
TypePanel
Format96-array Plate
SpeciesHuman
Unit SizeEach
Contents & Storage
Contents: Two 96-well plates each containing the 96-assay panel and two optical covers

Store at room temperature.

Frequently asked questions (FAQs)

I'm using the TaqMan hPSC Scorecard Panel. How do I load the samples onto a 384-well plate if I don't have a multichannel pipette?

You can load samples using a single channel pipette, but this method is time-consuming and may increase pipetting error. We strongly recommend that you use an 8- or 16-channel multichannel pipette. You also can dispense samples using automated systems, with the understanding that additional sample will be required to compensate for the dead volume.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

Why should I set up cDNA synthesis in 8 wells of a 96-well plate or in 8-well PCR strips when using the TaqMan hPSC Scorecard Panel?

Setting up your cDNA synthesis in 8 wells of a 96-well plate or in 8-well PCR strips facilitates sample loading of the 96-well and 384-well hPSC Scorecard Panel plates.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

Can I analyze somatic non-pluripotent primary cells with the TaqMan hPSC Scorecard Panel?

Somatic non-pluripotent primary cells, such as the parental lines used for iPSC generation, are not pluripotent in nature and their scores will be low. However, the expression of lineage markers will largely rely on homogeneity of the cells. Note that the markers in the panel are designed to evaluate early germ layer specification and not any particular terminally differentiated state.

Does the reprogramming method affect the TaqMan hPSC Scorecard analysis results?

Minor differences in gene expression profiles are sometimes observed based on the reprogramming method, but in general the hPSC Scorecard analysis results do not change significantly for lines derived using various reprogramming methods. This was tested with ESC and iPS derived using episomal or Sendai-based reprogramming systems before and after seven days of spontaneous differentiation. Cells were grown in KSR-based media on irradiated MEFs prior to removal of FGF for EB formation.

Will the presence of Sendai virus affect my TaqMan hPSC Scorecard analysis results?

The presence or absence of Sendai virus in established iPSC clones does not have an impact on pluripotency and hence on TaqMan hPSC Scorecard analysis results.

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