Janus Green B is a stain that interacts with DNA and has been used for histology studies including mitochondrial staining, in which its oxidation and reduction reveals alterations to the electron transfer chain. The stain has been utilized in studies for effective and rapid staining of insect chordotonal organs and peripheral nerves, where the nerves appear dark blue in comparison to non stained tissue. With the use of Janus Green B, rabbit lymphatic vessel studies demonstrate that threadlike structures within the lymphatic vessels, known as Bonghan ducts, contain a high density of mitochondria in their cells.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Janus Green B is a stain that interacts with DNA and has been used for histology studies including mitochondrial staining, in which its oxidation and reduction reveals alterations to the electron transfer chain. The stain has been utilized in studies for effective and rapid staining of insect chordotonal organs and peripheral nerves, where the nerves appear dark blue in comparison to non stained tissue. With the use of Janus Green B, rabbit lymphatic vessel studies demonstrate that threadlike structures within the lymphatic vessels, known as Bonghan ducts, contain a high density of mitochondria in their cells.
Solubility
Soluble in water (20 mg/ml at 20°C), ethanol, and slightly soluble in alcohol.
Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.
RUO – Research Use Only
Huang CZ, et al. Interactions of Janus Green B with double stranded DNA and the determination of DNA based on the measurement of enhanced resonance light scattering.Analyst.,2000,125(7), 1267-72.
Yack JE. Janus Green B as a rapid, vital stain for peripheral nerves and chordotonal organs in insects.J Neurosci Methods.,1993,49(1-2), 17-22.