Glutaraldehyde is used for cross-linking proteins and polyhydroxy materials. Also used as a fixative for tissues and as an amine-reactive homobifunctional crosslinker. It is also used in coupling reaction of biotin hydrazides to nucleic acids which is useful for conjugating fluorescent hydrazides and hydroxylamines to DNA and for stabilization ofproteins on agarose beads,activation of polystyrene and glass, for immobilization of antibodies and antigens.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Glutaraldehyde is used for cross-linking proteins and polyhydroxy materials. Also used as a fixative for tissues and as an amine-reactive homobifunctional crosslinker and as a disinfectant for medical equipment. It is also used in coupling reaction of biotin hydrazides to nucleic acids which is useful for conjugating fluorescent hydrazides and hydroxylamines to DNA and for stabilization ofproteins on agarose beads,activation of polystyrene and glass, for immobilization of antibodies and antigens. It is a disinfectant, which is rapidly effective against vegetative forms of Gram-positiveand Gram-negative bacteria.
Solubility
Miscible with water, ethanol, benzene, ether, acetone, dichloromethane, ethylacetate, isopropanol, n-hexane and toluene.
Notes
Heat and air sensitive. Incompatible with strong acids, strong bases and strong oxidizing agents.
RUO – Research Use Only
General References:
- For use in the Robinson synthesis of pseudopelletierine, see Acetone-1,3-dicarboxyl ic acid, A13742.
- Reaction with (R)-(-)-2-Phenyl glycinol, A19030, gives an intermediate for asymmetric synthesis of alkaloids: Org. Synth. Coll., 9, 176 (1998).
- Cho, K. L.; Hill, A. J.; Caruso, F.; Kentish, S. E. Chlorine Resistant Glutaraldehyde Crosslinked Polyelectrolyte Multilayer Membranes for Desalination. Adv. Mater. 2015, 27 (17), 2791-2796.
- Vikram, A.; Bomberger, J. M.; Bibby, K. J. Efflux as a Glutaraldehyde Resistance Mechanism in Pseudomonas fluorescens and Pseudomonas aeruginosa Biofilms. Antimicrob. Agents Chemother. 2015, 59 (6), 3433-3440.