GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

CRISPR-STOP is a method of inserting STOP codon sequences to generate knockouts.

Please refer to the following article: CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations.

Find additional tips, troubleshooting help, and resources within our Genome Editing Support Center.

For transfecting of the Cas9 protein, we would recommend using the Neon transfection system or Lipofectamine CRISPRMAX Cas9 Transfection Reagent. For transfection of mRNA, we would recommend using Lipofectamine MessengerMAX Transfection Reagent. For transfection of CRISPR vectors, we would recommend using Lipofectamine 3000 Transfection reagent.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Our CRISPR products are optimized for mammalian systems, and have not been optimized for plant systems. However, we know researchers are doing that kind of work. While we have not tested our CRISPR products for plants, our new protein format would be ideal since it does not need to be translated and transcribed in the cell, so no plant-specific promoters required.

Invitrogen GeneArt Precision TALs, in addition to gene deletion, down-regulation and integration, can also be used for gene activation. Additionally, the system is based on a protein-DNA system, in contrast to CRISPR, which is based on a RNA-DNA system. TALs can be used to target any gene in any cell, including mammalian, bacterial, yeast, plants, insect, stem cells and zebrafish. Lastly, off-target effects are low when using the TAL system. Please refer to the following paper (http://www.sciencedirect.com/science/article/pii/S016816561500200X) where the authors compared TALs and CRISPR technology.

Here are possible reasons and solutions:

- Single-stranded (ss) oligonucleotides designed incorrectly; Make sure that each ss oligonucleotide contains the 5 nucleotides on the 3′ end required for cloning into the Invitrogen GeneArt CRISPR Nuclease Vector: ie., Top strand includes GTTTT on the 3′ end; Bottom strand includes CGGTG on the 3′ end.

- Double-stranded (ds) oligonucleotides were degraded; Store the 5 nM ds oligonucleotide stock in 1X Oligonucleotide Annealing Buffer. Avoid repeated freeze/thaw cycles; aliquot the 5 nM ds oligonucleotide stock and store at -20°C.

- Oligonucleotide annealing reaction inefficient; Ensure that the annealing reaction was performed as directed. If ambient temperature is >25°C, incubate the annealing reaction in a 25°C incubator.