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Invitrogen™
Amplex™ Red Glucose/Glucose Oxidase Assay Kit
The Amplex™ Red Glucose/Glucose Oxidase Assay Kit provides a sensitive and simple method for detecting d-glucose or glucose oxidase usingRead more
| Catalog Number | Quantity |
|---|---|
| A22189 | 500 assays |
Catalog number A22189
Price (BRL)
3.414,14
Each
Quantity:
500 assays
Price (BRL)
3.414,14
Each
The Amplex™ Red Glucose/Glucose Oxidase Assay Kit provides a sensitive and simple method for detecting d-glucose or glucose oxidase using a fluorescence microplate reader or fluorometer.
See our complete line of Fluorescence Microplate assays.
• Detect concentrations as low as 3 μM d-glucose or 0.05 mU/mL of glucose oxidase
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference
The Amplex™ Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) is a colorless, stable, and extremely versatile peroxidase substrate. Because peroxidase- and glucose oxidase-mediated reactions can be coupled, it is possible to measure glucose oxidase activity or the release of glucose by any glucosidase enzyme—for instance, β-glucosidase and glucocerebrosidase—in either a continuous or discontinuous assay. Because of the ability to couple reactions, this assay has been demonstrated to be useful for the quantification of glucose levels in foods, fermentation media, and bodily fluids.
In the assay, glucose oxidase reacts with d-glucose to form d-gluconolactone and hydrogen peroxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide then reacts with the Amplex™ Red reagent in a 1:1 stoichiometric ratio to generate the red-fluorescent product resorufin.
Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.
Use Amplex™ Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex™ Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex™ UltraRed Reagent (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex™ Red/UltraRed Stop Reagent (Cat. No. A33855). The Amplex™ Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours. Custom assay design and packaging are also available.
See our complete line of Fluorescence Microplate assays.
• Detect concentrations as low as 3 μM d-glucose or 0.05 mU/mL of glucose oxidase
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference
The Amplex™ Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) is a colorless, stable, and extremely versatile peroxidase substrate. Because peroxidase- and glucose oxidase-mediated reactions can be coupled, it is possible to measure glucose oxidase activity or the release of glucose by any glucosidase enzyme—for instance, β-glucosidase and glucocerebrosidase—in either a continuous or discontinuous assay. Because of the ability to couple reactions, this assay has been demonstrated to be useful for the quantification of glucose levels in foods, fermentation media, and bodily fluids.
In the assay, glucose oxidase reacts with d-glucose to form d-gluconolactone and hydrogen peroxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide then reacts with the Amplex™ Red reagent in a 1:1 stoichiometric ratio to generate the red-fluorescent product resorufin.
Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.
Use Amplex™ Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex™ Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex™ UltraRed Reagent (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex™ Red/UltraRed Stop Reagent (Cat. No. A33855). The Amplex™ Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours. Custom assay design and packaging are also available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), 96-well plate
Quantity500 assays
Shipping ConditionRoom Temperature
For Use With (Application)Glucose/Glucose Oxidase Assay
For Use With (Equipment)Microplate Reader, Spectrophotometer, Fluorometer
Product LineAmplex
Product TypeRed Glucose/Glucose Oxidase Assay Kit
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
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Lot #Certificate TypeDateCatalog Number(s)
3143060Certificate of AnalysisApr 04, 2025A22189
2935400Certificate of AnalysisJun 03, 2024A22189
2885717Certificate of AnalysisApr 05, 2024A22189
2761154Certificate of AnalysisDec 05, 2023A22189
2714561Certificate of AnalysisAug 17, 2023A22189
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SDSScientific Resources
Product Information
Molecular Probes® Handbook
Frequently asked questions (FAQs)
The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.
The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (21)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Development of a long-acting insulin analog using albumin fusion technology.
Journal:Diabetes
PubMed ID:15616036
The primary therapeutic goal for the treatment of diabetes is maintenance of a long-term, near-normoglycemic condition and prevention of the onset or progression of the complications associated with the disease. Although several analogs of human insulin have been developed, the currently prescribed long-acting insulin analogs do not provide a stable
Perturbational profiling of a cell-line model of tumorigenesis by using metabolic measurements.
Journal:Proc Natl Acad Sci U S A
PubMed ID:15840712
'Weinberg and coworkers have used serial transduction of a human, primary fibroblast cell line with the catalytic domain of human telomerase, large T antigen, small T antigen, and an oncogenic allele of H-ras to study stages leading toward a fully transformed cancerous state. We performed a three-dimensional screening experiment using
Irs1 and Irs2 signaling is essential for hepatic glucose homeostasis and systemic growth.
Journal:J Clin Invest
PubMed ID:16374520
'Insulin receptor substrates, including Irs1 and Irs2, integrate insulin and IGF receptor signals with heterologous pathways to coordinate growth and metabolism. Since Irs2 is thought to be especially important in hepatic nutrient homeostasis, we deleted Irs2 [corrected] from hepatocytes of WT mice (called LKO) or genetically insulin-resistant Irs1-/- mice (called
Stabilization of enzymes in silk films.
Journal:Biomacromolecules
PubMed ID:19323497
'Material systems are needed that promote stabilization of entrained molecules, such as enzymes or therapeutic proteins, without destroying their activity. We demonstrate that the unique structure of silk fibroin protein, when assembled into the solid state, establishes an environment that is conducive to the stabilization of entrained proteins. Enzymes (glucose
Inhibition of mitochondrial pyruvate transport by zaprinast causes massive accumulation of aspartate at the expense of glutamate in the retina.
Journal:
PubMed ID:24187136
'Transport of pyruvate into mitochondria by the mitochondrial pyruvate carrier is crucial for complete oxidation of glucose and for biosynthesis of amino acids and lipids. Zaprinast is a well known phosphodiesterase inhibitor and lead compound for sildenafil. We found Zaprinast alters the metabolomic profile of mitochondrial intermediates and amino acids
21 total citations