MagMAX™ DNA Multi-Sample Ultra Kit (with extra proteinase K and DNA binding beads)
MagMAX™ DNA Multi-Sample Ultra Kit (with extra proteinase K and DNA binding beads)
Applied Biosystems™

MagMAX™ DNA Multi-Sample Ultra Kit (with extra proteinase K and DNA binding beads)

Note: This product is identical to the MagMAX™ DNA Multi-Sample Ultra Kit (Cat. No. A25597) except that it comes withRead more
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Catalog NumberQuantity
A25919500 Preps
A259202,500 preps
Catalog number A25919
Price (USD)
3,545.00
Each
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Quantity:
500 Preps
Price (USD)
3,545.00
Each
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Note: This product is identical to the MagMAX™ DNA Multi-Sample Ultra Kit (Cat. No. A25597) except that it comes with additional proteinase K and DNA Binding Beads, which some customers find useful for their specific applications.

The MagMAX™ DNA Multi-Sample Ultra Kit is optimized to isolate genomic DNA (gDNA) from a variety of samples, such as whole blood, buccal cells, saliva, urine, blood cards, mouth rinse, and tissue. gDNA purified with the kit is ideal for qPCR applications on sensitive, scalable platforms such as the QuantStudio™ 12K Flex System with OpenArray™ Block (with AccuFill™ System). The magnetic bead–based purification format allows you to easily scale from processing 12 to 500 samples a day, making it the ideal choice for pharmacogenomics (PGx) studies.

Features of the MagMAX™ DNA Multi-Sample Ultra Kit include:
• Streamlined protocols for numerous non-invasive biological samples
• Optimized to provide DNA yields suitable for OpenArray™ and Ion AmpliSeq™ applications
• Packaged for high-throughput needs

One Kit for a Diverse Set of Sample Types
Why purchase multiple purification kits to isolate gDNA from different sample types? The MagMAX™ DNA Multi-Sample Ultra Kit eliminates the headache of ordering these extra kits because it has been optimized for use with a variety of biological samples. The kit is designed for high-throughput isolation of gDNA from 1 buccal swab, 50 to 350 μL of whole blood, up to 400 μL urine (without sample concentration), up to 150 μL saliva, 1-5 mL mouth rinse, blood spot cards, and mammalian tissue samples (up to 10 mg). The protocols and magnetic bead format make the kit fully amenable to automation.

*Please note that the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor with a 24 Deep-well Head is recommended for sample volumes greater than 50 μL.

Benefits of MagMAX™ Magnetic Bead–based Purification
Magnetic beads offer many benefits compared to other technologies for isolating gDNA. Beads bind gDNA more efficiently than glass fiber filters, resulting in higher and more consistent gDNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.

Accessory Products
Proteinase K (Cat. No. A25561), DNA Binding Beads (Cat. No. A25562), Wash Solution 1 Concentrate (Cat. No. AM8504), and Wash Solution 2 Concentrate (Cat. No. AM8640) are components of the kit that may also be purchased separately.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)
High-throughput CompatibilityHigh-throughput Compatible, Manual Protocols, Automated Protocols
No. of Reactions500 Preps
Product LineMagMAX™
Product TypeDNA Multi-Sample Ultra Kit
Quantity500 Preps
Sample TypeBlood, Buccal Samples, Cells, Liquid Samples (e.g. Serum), Tissue
TargetGenomic DNA
FormatKit
Unit SizeEach
Contents & Storage
• DNA Binding Beads, 2 x 8 mL (store at 2°C to 8°C, DO NOT FREEZE)
• DNA Elution Buffer 1, 1 x 25 mL (store at room temperature)
• DNA Elution Buffer 2, 1 x 25 mL (store at room temperature)
• Wash Solution 1 Concentrate, 1 x 80 mL* (store at room temperature)
• Wash Solution 2 Concentrate, 1 x 162 mL* (store at room temperature)
• Multi-Sample DNA Lysis Buffer, 1 x 100 mL (store at room temperature)
• Nuclease-Free Water, 1 x 100 mL (store at room temperature)
• PK Buffer, 1 x 96 mL (store at room temperature)
• Proteinase K, 2 x 4 mL (store at -5° to -30°C)
• Ribonuclease A (RNase A), 1 x 1.25 mL (store at -5° to -30°C)
*after addition of alcohol
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Frequently asked questions (FAQs)

Does the PowerUp SYBR Green Master Mix (Cat. No. A25741, A25742, A25743, A25776, A25777, A25778, A25779, A25780, A25919) contain uracil-DNA glycolase?

This master mix has a heat-labile UDG. It is completely and irreversibly inactivated after 10 minute incubation at 50°C.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What type of samples is the MagMAX DNA Multi-Sample Ultra Kit (Cat. No. A25597) optimized for?

We have optimized protocols for extracting DNA from buccal swabs, whole blood, blood cards (Whatman FTA), oral rinse, saliva, and urine samples. In addition, the kit can be used to process vaginal microbiota samples (e.g., ThinPrep, Surepath) for gDNA extraction from yeast, bacteria (Gram positive/negative), viruses, and protozoa with the addition of additional enzymatic steps.

In the MagMAX DNA Multi-Sample Ultra Kit protocol, why do blood samples appear cloudy after adding the Multi-Sample DNA Lysis Buffer?

On rare occasions, the sample may turn cloudy after adding the Multi-Sample DNA Lysis Buffer. The sample will clear up when isopropanol is added. This will not have any impact on the yield or quality of the DNA.

Can heparin be used to collect blood samples for the MagMAX DNA Multi-Sample Ultra Kit?

Heparin is not recommended as an anti-coagulant since it can cause inhibition of PCR reactions. Instead, we strongly recommend collecting blood samples in EDTA or sodium citrate.

What is the best way to remove the buccal swabs from the MagMAX DNA Multi-Sample Ultra Kit lysate?

We recommend transferring the lysate to a new MagMAX Express-96 Deep Well Plate (Cat. No. 4388476). This option eliminates contamination risks and saves time. To transfer lysates, set a multi-channel micropipetor to ˜300 µL and transfer one row at a time. Each well should contain 200-250 µL after transfer.

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