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Ion 16S™ Metagenomics Kit
The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive and broad-range analyses of mixed microbial populations using the IonRead more
| Catalog Number | Quantity |
|---|---|
| A26216 | 100 reactions |
Catalog number A26216
Price (EUR)
1.284,00
Each
Quantity:
100 reactions
Price (EUR)
1.284,00
Each
The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive and broad-range analyses of mixed microbial populations using the Ion Torrent™ semiconducter sequencing workflow. The kit permits PCR amplification of hypervariable regions of the 16S rDNA gene from bacteria. The amplified fragments can then be sequenced using the Ion PGM™ Sequencing 400 Kit on the Ion PGM™ platform and analyzed using the Ion 16S™ metagenomics analyses module within the Ion Reporter™ software.
Comprehensive
The kit includes 2 sets of primers that can be used to amplify the corresponding hypervariable regions of the 16S rDNA gene in bacteria:
- Primer set V2-4-8
- Primer set V3-6,7-9
These comprehensive primer sets allow for accurate detection and identification of a broad range of bacteria down to the genus or species level. The primer sets are paired with Environmental Master Mix v2.0 that is optimized to tolerate high levels of PCR inhibitors and amplify targets from complex samples such as environmental, food, tissue and other challenging samples. Additionally, the kit includes a positive control for troubleshooting experiments.
Flexible
The primer sets are provided as two pools. Each pool can be used individually or in combination to allow for flexibility in experimental set up. When combined with the Ion Xpress™ Barcode Adapters kits, multiple polymicrobial samples can be combined in a single sequencing workflow.
Complete Workflow
Sequences generated using the Ion 16S™ Metagenomics Kit can be directly analyzed using the Ion 16S™ metagenomics analyses module within the Ion Reporter™ software enabling a rapid and semi-quantitative assessment of complex microbial samples.
Note: This kit is not compatible with the Ion Library Quantitation Kit (Cat. No. 4468802). For accurate quantitation of amplicons generated using this kit, use the Ion Universal Library Quantitation Kit (Cat. No. A26217).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodIon Torrent Sequencing, Primer-Probe Detection
Sample TypeDNA
For Use With (Equipment)Ion GeneStudio™ S5 Series Sequencer
For Use With (Application)Sequencing
No. of Reactions250 Reactions
PolymeraseTaq DNA Polymerase
Product LineIon Torrent™
Quantity100 reactions
SpeciesAll Species
Target Organism ClassAll Bacteria
Test TimeFast
Unit SizeEach
Contents & Storage
• DNA dilution buffer (7 mL)
• 2 tubes 2X environmental master mix (0.8 mL per tube)
Store the above at 2-8°C.
• E. coli DNA control (40 μL)
• Negative control water (1 mL)
• 16S primer set V2-4-8 (300 μL)
• 16S primer set V3-6,7-9 (300 μL)
Store the above at -5 to -30°C.
• 2 tubes 2X environmental master mix (0.8 mL per tube)
Store the above at 2-8°C.
• E. coli DNA control (40 μL)
• Negative control water (1 mL)
• 16S primer set V2-4-8 (300 μL)
• 16S primer set V3-6,7-9 (300 μL)
Store the above at -5 to -30°C.
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Lot #Certificate TypeDateCatalog Number(s)
1810014Certificate of AnalysisDec 12, 2018A26216
1702011Certificate of AnalysisJul 26, 2017A26216
1502004Certificate of AnalysisJul 21, 2017A26216
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
The Ion 16S Metagenomics Kit is designed for rapid analyses of polybacterial samples using Ion Torrent Sequencing technology. The kit includes two primer sets that selectively amplify the corresponding hypervariable regions of the 16S region in bacteria:
- Primer set V2-4-8
- Primer set V3-6, 7-9
Sequence the amplified fragments on the Ion PGM System and analyze the results with the Ion Reporter software Ion 16S Metagenomics Kit analyses module. The combination of the two primer pools allows for sequence-based identification of a broad range of bacteria within a mixed population.
Yes. You can purchase the TaqMan Environmental Master Mix 2.0 (Cat. No. 4396838).
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center
For each sample, the protocol involves two PCR reactions, one with primer set V2-4-8 and one with primer set V3-6.7. However, the kit contains sufficient master mix for 100 reactions and each of the two primer pools has sufficient volume for 100 reactions. Thus, the kit contains sufficient reagents for 100 reactions/50 samples.
Note: 100 reactions is dependent upon on the master mix, which is the limiting reagent. As far as primers are concerned, there is sufficient volume to process another 50 samples.
It is sufficient for 100 PCR reactions.
No, the Ion 16S Metagenomics Kit utilizes 400 bp reads, so it is incompatible with the Ion 540 Kit - Chef, which only yields 200 bp reads.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.