Ion 530™ Chip Kit
Ion 530™ Chip Kit
Ion Torrent™

Ion 530™ Chip Kit

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The Ion 530 Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5 and IonRead more
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Catalog NumberQuantity
A277648 chips
Catalog number A27764
Price (USD)
6,880.00
Each
-
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Quantity:
8 chips
Price (USD)
6,880.00
Each
Add to cart
The Ion 530 Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5 and Ion S5 XL Sequencing Systems. The Ion 530 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times of less than 2.5 hours for 200 bp sequencing.

The Ion 530 Chip offers:

• Sample tracking functionality from an integrated barcode
• A rapid run time of only 2.5–4 hours, generating 15–20 million reads
• Read lengths up to 600 bp to support a variety of research applications, including targeted gene sequencing and microbial sequencing
• Easy handling and loading of templated products for sequencing
• Compatibility with current library preparation methods

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL systems.
Use this kit with the Ion 510 & Ion 520 & Ion 530 Kit-Chef, the Ion 520 & Ion 530 ExT Kit-Chef, and the Ion 520 & Ion 530 Kit-OT2.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Ion GeneStudio S5 System, Ion S5™ XL System
Green FeaturesSustainable disposal
Number of Reads per Chip15-20M
Product TypeChip Kit
Quantity8 chips
Shipping ConditionRoom Temperature
Product LineIon 530™
Unit SizeEach
Contents & Storage
Store at room temperature

Frequently asked questions (FAQs)

How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?

The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

For the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay, what are the analysis workflows in Ion Reporter Software?

There are two analysis workflows available:

1. Oncomine TagSeq Lung v2 Liquid Biopsy - w2.0 - Single Sample: Detects and annotates low-frequency variants including SNPs/Indels (down to 0.1% limit of detection), Fusions, and CNVs from targeted nucleic acid libraries (DNA & RNA) from the Oncomine Lung Cell‑Free Total Nucleic Acid Research Assay. This is compatible with DNA & RNA purified from cell-free total nucleic acids.

2. Oncomine TagSeq Lung v2 Tumor - w2.0 - Single Sample: Detects and annotates low-frequency variants including SNPs/Indels (down to 0.5% limit of detection), fusions, and CNVs from targeted nucleic acid libraries (DNA & RNA) from the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay. Due to deamination events caused by the FFPE process, the minimum alternative allele frequency is set to 0.3%. This makes it compatible with DNA & RNA purified from FFPE tumor tissue as well as fresh frozen tumor tissue.

Are there any BED files for the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay?

Yes, there is a specific BED file and Hotspot file for the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay. Please contact your local Field Application Specialist (FAS) or Clinical Application Consultant (CAC) to request the BED files.

What is the LOD (limit of detection) for the Oncomine Lung Cell-Free Total Nucleic Acid (cfNA) Research Assay?

Through the use of Tag Sequencing technology, low limits of detection (LOD) can be achieved for different variant types*:

- For SNVs/short indels, an LOD of 0.1% can be achieved with sensitivity of ˜90% and specificity of >99%
- For fusions & MET exon skipping, an LOD of 1% can be achieved with sensitivity of >90% and specificity of >99%
- For MET CNV target, detection as low as 1.2-fold amplification can be achieved with sensitivity of >90% and specificity of >99%

*Sensitivity and specificity for each variant type were determined using a collection of contrived positive samples and cfNA isolated from normal healthy donors.

Does the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay use AmpliSeq technology?

No, this assay uses Tag Sequencing technology. Cell-free DNA (cfDNA) and cell-free RNA (cfRNA) are found at extremely low concentrations in the plasma fraction of whole blood. Because of this low prevalence, Taq Sequencing technology is utilized in this assay. The technology attaches unique molecular tags to the gene-specific primers. After amplification, the tagged molecules are grouped based on the tags. Groups containing the same mutant variant 80% of the time or greater will be called positive. Using the Tag technology, groups that contain random errors generated through the library construction/sequencing process are removed.

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