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rhLaminin-521
Recombinant human Laminin 521 (rhLaminin-521) is a recombinant human protein that provides a defined surface for feeder-free culture of humanRead more
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| Catalog Number | Quantity |
|---|---|
| A29248 | 100μg |
| A29249 | 1 mg |
Catalog number A29248
Price (USD)
119.65
Online Exclusive
125.00Save 5.35 (4%)
Each
-
Quantity:
100μg
Price (USD)
119.65
Online Exclusive
125.00Save 5.35 (4%)
Each
Recombinant human Laminin 521 (rhLaminin-521) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium, rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 also provides optimum reprogramming efficiency of human somatic cells as well as human pluripotent stem cell survival during feeder-dependent to feeder-free transitions when used in conjunction with Essential 8 Medium. For a kit that combines the medium and substrate, see the Essential 8™ Adaptation Kit.
rhLaminin-521 is:
• Robust—transition cells between feeder-dependent and feeder-free culture systems with minimal effort
• Efficient—easily reprogram human fibroblasts to pluripotent stem cells
• Physiologically relevant—expand cells with minimal differentiation
• Scalable—passage stem cell lines as single cells with no impact to genetic stability
Trouble-free transition
When used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.
Efficient reprogramming
rhLaminin-521 has been shown to increase the number of colonies obtained during reprogramming of human fibroblasts, which can be important for in vitro disease modeling.
Confident expansion
rhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.
Easy scale-up
rhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.
rhLaminin-521 is:
• Robust—transition cells between feeder-dependent and feeder-free culture systems with minimal effort
• Efficient—easily reprogram human fibroblasts to pluripotent stem cells
• Physiologically relevant—expand cells with minimal differentiation
• Scalable—passage stem cell lines as single cells with no impact to genetic stability
Trouble-free transition
When used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.
Efficient reprogramming
rhLaminin-521 has been shown to increase the number of colonies obtained during reprogramming of human fibroblasts, which can be important for in vitro disease modeling.
Confident expansion
rhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.
Easy scale-up
rhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeStem Cells
Culture TypeStem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
Purity or Quality GradehESC-Qualified
Quantity100μg
Shelf Life24 Months
Shipping ConditionDry Ice
SourceXeno-Free
ClassificationXeno-free
Product TypeExtracellular Matrix
pH6 to 8
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -20°C) and protect from light.
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Figures
iPSC imaged on EVOS M5000 microscope
Human ESCs maintain expression of self-renewal factors
PSCs maintain normal karyotype
Human ESCs maintain pluripotency
Optimum recovery of PSCs during transition to Essential 8 Medium
iPSC imaged on EVOS M7000 microscope
Human ESCs maintain expression of self-renewal factors
iPSC imaged on EVOS M7000 microscope
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Lot #Certificate TypeDateCatalog Number(s)
3121035Certificate of AnalysisMay 14, 2025A29249
3101144Certificate of AnalysisApr 09, 2025A29249
80398Certificate of AnalysisMar 10, 2025A29248
80406Certificate of AnalysisMar 10, 2025A29248
2875548Certificate of AnalysisJan 07, 2025A29249
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Product Information
Frequently asked questions (FAQs)
The appropriate alternative for rhLaminin-521 (Cat. No. A29249) would be either Laminin Mouse Protein, Natural (Cat. No. 23017015) or Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A14700).
Find additional tips, troubleshooting help, and resources within our Stem Cell Culture Support Center.
iPSC-derived neurons have been cultured successfully on Poly-D-Lysine with a secondary coating of mouse and human laminin.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of CD34+ blood cells. For CD34+ cells, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 39-44). On Day 3, you can utilize rhVTN-N (Cat. No. A14700), Geltrex (Cat. No. A1413302), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, reprogrammed CD34+ cells should be fed every-other-day with StemFlex Medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of both neonatal and adult human dermal fibroblasts. For fibroblasts, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 16-20). On Day 7, you may use rhVTN-N (Cat. No. A14700), Geltrex matrix (Cat. No. A1413302), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, we recommend that you feed reprogrammed fibroblasts every-other-day with StemFlex Medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We have not yet evaluated the Geltrex matrix system for clonal expansion in the presence of StemFlex Medium. However, rhLaminin-521 does provide optimal survival of cells following single-cell passaging and thus this matrix is recommended for such critical applications.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.