High Resolution Melt Software v3.2

Name: High Resolution Melt Software v3.2
SKU: A30150
Price: 2270.00 USD

High Resolution Melt Software v3.2 is an all-new and improved tool for High Resolution Melting (HRM) analysis. HRM analysis isRead more

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Catalog Number	Content And Storage
A30150
A29881

2 Options

Catalog number A30150

Price (USD)

2,270.00

Each

Add to cart

Price (USD)

2,270.00

Each

Add to cart

High Resolution Melt Software v3.2 is an all-new and improved tool for High Resolution Melting (HRM) analysis. HRM analysis is a post-PCR analysis method used to identify variation in nucleic acid sequences. The method is based on detecting small differences in PCR melting (dissociation) curves. It is enabled by high-brightness, dsDNA-binding dyes used in conjunction with real-time PCR instrumentation that has precise temperature ramp control, advanced data capture capabilities, and access to software designed specifically for HRM analysis. High Resolution Melt Software v3.2 brings HRM to the QuantStudio 1, QuantStudio 3 and 5, StepOne and StepOnePlus Real-Time PCR Systems, and the 7500 Fast Real-Time PCR System, and contains features that make fast and accurate HRM results more accessible than ever.

Download High Resolution Melt (HRM) Software (license key required) ›

Key product features:

• Minimal user input to achieve results—software automatically applies default/saved analysis settings upon opening an experiment file
• Easy recall—new Analysis Settings Library saves settings for future experiments
• Greater accuracy—clustering algorithm accurately distinguishes control and variant genotypes
• Increased sensitivity—software allows input of expected number of clusters for difficult-to-detect SNPs (class 4)
• Ability to achieve more in one run—separate analysis of multiple assays run on a single plate
• Shortcuts in experiment setup—direct copy and paste functions with Excel; well information and plate layout clearly displayed in Plate View

Minimize user input to achieve results and easily recall analysis settings
High Resolution Melt Software v3.2 automatically applies default or saved analysis settings upon opening an experiment file, which allows the user to achieve results with minimal input. It also includes a new Analysis Settings Library, allowing the user to save optimized settings and easily recall them for use in future experiments.

Realize greater accuracy and increase your sensitivity
The v3.2 software utilizes an improved clustering algorithm that helps to accurately distinguish control and variant genotypes, and allows the user to input the expected number of clusters for increased sensitivity for difficult SNP genotyping.

Achieve more in one run
With the v3.2 software, you now have the ability to conduct separate analyses of multiple assays run on a single plate.

Get your experiments up and running fast with shortcuts in setup
Users of High Resolution Melt Software v3.2 can copy and paste directly from Excel spreadsheets to set up experiments quickly. Plate View also clearly displays well information and the plate layout to ease plate setup.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)7500 Fast System, QuantStudio™ 1, QuantStudio™ 3, QuantStudio™ 5, StepOnePlus™ System, StepOne™ System

Quantity1 license

Shipping ConditionRoom Temperature

Unit SizeEach

Contents & Storage

Includes One License Key for 1 User

Frequently asked questions (FAQs)

What ramp mode should I use for HRM experiments using the QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems?

We recommend using Continuous mode (set to 0.025 degrees C/sec) for HRM experiments. You can also choose “Number of Data Points per Degree”, but if you do so, make sure to collect at least 10 points per degree so that there is enough data for analysis. When in Continuous mode, the instrument uses a fixed ramp rate for the melt step and captures as many data points as possible within the time permitted. When in Data Point Per Degree mode, the instrument adjusts the step and hold for the melt step to achieve the specified number of data points.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What calibrate plate can I use for High Resolution Melt (HRM) analysis on the QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems?

We recommend using our MeltDoctor HRM Calibration plates. Use the plate type that matches the block on your QuantStudio 3 or QuantStudio 5 Real-Time PCR System:

0.1 mL, 96-Well: Cat. No. 4425618
384-Well: Cat. No. 4425559
0.2 mL, 96-well: Cat. Nos. 4425562 and 4425557 (Note: We do not have a 0.2 mL calibration plate, but you can make one by combining the MeltDoctor HRM Calibration Standard with the MeltDoctor HRM Reagent Kit)

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Are the QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems compatible with High Resolution Melt (HRM) experiments?

Yes, you can perform HRM experiments on the QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems. The data can either be analyzed in the HRM v3.1 Software (Cat. No. A30150), or in the Thermo Fisher Cloud HRM Analysis Application. Please see here (https://tools.thermofisher.com/content/sfs/manuals/MAN0014394_HighResMeltExperiment_GSG.pdf) for the user manual (with Cloud workflow). Note: QuantStudio 3/QuantStudio 5 Real-Time PCR System firmware version 1.1 or later is required to perform HRM experiments.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What are the license renewal requirements for the High Resolution Melt (HRM) software?

If you are using the QuantStudio 3/QuantStudio 5 Real-Time PCR Systems, 7500 Real-Time PCR System (with SDS v2.0.5 or later), or StepOne/StepOnePlus Real Time PCR Systems, you can purchase High Resolution Melt (HRM) Software v3.1 (https://www.thermofisher.com/order/catalog/product/A30150?ICID=search-product). Once activated, this software will not require annual renewal. If you have the older HRM software v3.0.1 (for use with StepOne Plus, StepOne or the 7500 Fast (SDS v2.0.5 or later) Real-Time PCR System, it will require license renewal on an annual basis. For use on the 7900HT Fast Real-Time PCR System, please use HRM Software v2.0.1 (https://www.thermofisher.com/order/catalog/product/4397808?ICID=search-product). This software will require license renewal on an annual basis. When using either the ViiA 7, QuantStudio 6/QuantStudio 7 or QuantStudio 12K Flex Real-Time PCR Systems, the HRM software is already included in the instrument's application software, but requires activation of the HRM module within the instrument software by purchase of the license. This license requires an annual renewal.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Where can I find information regarding High Resolution Melt (HRM) software v3.1 and HRM software for use on the QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems?

For more information about HRM software v3.1 and HRM software for use on the QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems, please go here (http://tools.thermofisher.com/content/sfs/manuals/MAN0014394_HighResMeltExperiment_GSG.pdf).

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.