PSC Dopaminergic Neuron Differentiation Kit
PSC Dopaminergic Neuron Differentiation Kit
Gibco™

PSC Dopaminergic Neuron Differentiation Kit

The Gibco™ PSC Dopaminergic Neuron Differentiation Kit is a culture media system that enables the differentiation of human pluripotent stemRead more
Have Questions?
Catalog NumberQuantity
A31477011 kit
Catalog number A3147701
Price (USD)
3,765.00
Each
-
Add to cart
Quantity:
1 kit
Price (USD)
3,765.00
Each
Add to cart
The Gibco™ PSC Dopaminergic Neuron Differentiation Kit is a culture media system that enables the differentiation of human pluripotent stem cells (hPSCs) into functional midbrain dopaminergic neurons. Unlike other methods of differentiating hPSCs into dopaminergic neurons, which can be lengthy, ill-defined, and produce a biologically irrelevant phenotype, the PSC Dopaminergic Neuron Differentiation Kit allows you to differentiate hPSCs into authentic midbrain dopaminergic neurons with increased speed and unprecedented scalability, all while retaining proper midbrain phenotype throughout.

The kit is a three-step system used over 35 days, with components that are sequentially added to specify PSCs to the midbrain floor plate, expand floor plate progenitor cells, and differentiate floor plate progenitor cells into functional midbrain dopaminergic neurons. Each component is performance tested to ensure generation of the correct phenotype, and the midbrain dopaminergic neurons have been functionally tested for the ability to produce spontaneous action potential and release of dopamine upon depolarization.

Step 1: Specification of PSCs into midbrain floor plate cells
During central nervous system development, midbrain dopaminergic neurons are derived from a distinct population of cells termed midbrain floor plate cells, which are formed during days 21–28 of gestation and located along the ventral midline of the developing neural tube. Recent reports have focused on the importance of differentiating hPSCs into cells from this region, rather than using a more generic neural stem cell population, in order to create authentic midbrain dopaminergic neurons. The first step in the creation of dopaminergic neurons using the PSC Dopaminergic Neuron Differentiation Kit is the specification of hPSCs into midbrain-specified floor plate progenitor (FP) cells in complete Floor Plate Specification Medium.

Step 2: Create a bankable population of floor plate progenitor cells
Standard methods of creating midbrain dopaminergic neurons involve lengthy protocols that are continuous and not scalable. Using the PSC Dopaminergic Neuron Differentiation Kit, a workable bank of floor plate progenitor cells that maintain midbrain identity is created.

The ability to bank progenitor cells in this step enables:
• A significant increase in resultant DA neuron purity
• The ability to scale experiments beyond current limits
• The ability to distribute cells among peers or thaw for later use

Step 3: Differentiation of floor plate precursor cells to midbrain dopaminergic neurons
The final step of the PSC Dopaminergic Neuron Differentiation Kit protocol involves differentiation of floor plate progenitor cells into midbrain dopaminergic neurons. Dopaminergic neurons generated during this step have been shown to maintain midbrain identity, exhibit a gene expression profile equivalent to that of neurons generated using published protocols, and exhibit functionality as shown by spontaneous action potential and release of dopamine upon depolarization.

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity1 kit
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
• 1 x 5 mL Floor Plate Cell Specification Supplement
• 1 Floor Plate Cell Expansion Kit
• 1 x 10 mL Dopaminergic Neuron Maturation Supplement

Frequently asked questions (FAQs)

Is StemFlex Medium compatible with downstream differentiation kits available from Thermo Fisher Scientific?

Yes. We have seen compatibility with the following differentiation kits provided by Thermo Fisher Scientific: PSC Cardiomyocyte Differentiation Kit (Cat. No. A2921201), PSC Definitive Endoderm Induction Kit (Cat. No. A3062601), PSC Neural Induction Medium (Cat. No. A1647801), and PSC Dopaminergic Neuron Differentiation Kit (Cat. No. A3147701).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is cell density in Floor Plate Specification medium critical for Dopaminergic neuron differentiation?

Yes, cell density should be optimized to attain full confluency at day 7 in the specification medium to have an efficient differentiation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Dopaminergic Neuron Maturation medium be used to differentiate neural stem cells?

We have tested neural stem cells (NSCs) isolated from fetal tissue or derived from pluripotent stem cells (PSCs), and have seen that both populations can benefit from maturation medium (DMEM/F12 + Dopaminergic Neuron Maturation Supplement) to have nicely spread homogenous neurons with reduced progenitor population. Matured neurons can be further maintained in neurobasal medium supplemented with Dopaminergic Neuron Maturation Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer Dopaminergic Neuron differentiation service?

Yes. Please go here (https://www.thermofisher.com/us/en/home/life-science/stem-cell-research/stem-cell-services/stem-cell-differentiation-services.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link