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Catalog Number | Quantity |
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A32184 | 1 Library |
The award-winning Invitrogen™ LentiArray™ Human Druggable Genome Library targets 10,132 genes with up to 4 gRNA per gene target for a total of 40,512 gRNAs. Libraries are delivered as 50 μL of glycerol stock per gRNA and are also available as ready to use packaged lentivirus (Cat. No. A31948).
The LentiArray Human Druggable Genome Library is ideal for identifying potential therapeutic targets involved in the development and progression of disease. The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC).
Built to bring you success
LentiArray CRISPR libraries are constructed using gRNA designs produced using Thermo Fisher Scientific's proprietary CRISPR gRNA design algorithm. This algorithm incorporates the latest gRNA design research, as well as our extensive in-house experience, to produce the highest quality designs. The gRNA designs included in the LentiArray CRISPR libraries are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. For each gene target, up to four high-quality gRNAs are included to help ensure highly efficient knockout of the target gene in a wide array of cell types.
Design and execute your experiments without limitations
LentiArray CRISPR libraries are designed and constructed to give you complete control over your experimental design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA off of separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells.
The LentiArray Cas9 lentivirus construct expresses a human, codon-optimized Cas9 nuclease with a blasticidin-resistance gene under the control of the EF-1α (EFS) promoter. You can choose to co-infect your cells with both the LentiArray gRNA lentiviruses and the Cas9 lentivirus or just the LentiArray Cas9 lentivirus to establish a stable Cas9 expressing cell line. With LentiArray CRISPR libraries you have the freedom to design the experimental approach that best suits your model system and your screening goals.
Furthermore, to minimize restrictions to your experimental design, the gRNA lentiviruses that make up the LentiArray CRISPR libraries do not contain fluorescent markers. This allows reporters of any wavelength to be used with the libraries and expands your ability to perform multiplexed experiments. For challenging cell lines, the LentiArray gRNA lentiviruses contain a puromycin resistance gene that allows enrichment of the population of edited cells and drives stronger phenotypes.
Optimize your assay design and have full confidence in your results with LentiArray controls
High quality controls are the cornerstone of a successful screen. The LentiArray CRISPR libraries product line includes a suite of controls to help you quickly develop your assay and give you confidence in defining your hit criteria.
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TERMS OF USE
BEFORE PLACING AN ORDER OR USING THIS PRODUCT, PLEASE READ THE TERMS AND CONDITIONS SET FORTH BELOW. YOUR PURCHASE OF THIS PRODUCT SHALL CONSTITUTE ACKNOWLEDGMENT AND ACCEPTANCE OF THESE TERMS AND CONDITIONS.
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modifications of the Product) and Unmodified Derivatives (meaning any substances created by the purchaser which constitute an unmodified functional subunit or product expressed by the
Product) for RESEARCH USE ONLY, which, subject to the exclusions below, includes use to discover and develop any product, including therapeutic products, which may then be sold to third
parties, provided, however, that such products do not incorporate the Product, Progeny, Modifications, or Unmodified Derivatives.
No "Commercial Use" is allowed. Commercial Use means any and all uses of the Product, Progeny, Modifications or Unmodified Derivatives thereof, or any modified cells or organisms
created through use of the foregoing, including but not limited to:
(1) Sale, whether or not such sale is limited for use in research;
(2) Provision of a service to a third party;
(3) Use in any diagnostic, preventative, or therapeutic application;
(4) Use in any veterinary, livestock or agricultural application;
(5) Use in later stage development of therapeutics, diagnostics, prophylactics (e.g., hit-to-lead, lead optimization); and
(6) Manufacturing of a product for sale.
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remain in the possession of the purchaser, except for a transfer to a scientific collaborator or to a service provider, to perform services, solely on behalf of the purchaser.”
Notice to Purchaser: The purchase of this product conveys to the buyer limited, non-transferable rights under certain CRISPR-Cas technology owned and/or controlled by Toolgen, Inc . and licensed to Life Technologies Corporation, to use this product and components of this product only to perform internal research for the sole benefit of the buyer. The buyer may also use standard molecular biology techniques to make additional copies of any DNA components of this product for purposes of internal research for the sole benefit of the buyer. The buyer cannot sell or otherwise transfer (a) this product and its components (“CRISPR-Cas Product(s)”), or (b) materials, cells, or organisms made this CRISPR-Cas Product (“Derived Material(s)”) to a third party, or otherwise use this CRISPR-Cas Product or Derived Material for any Commercial Purpose or Development Purpose with the sole exception that buyer may transfer this CRISPR-Cas Product or Derived Material to (i) the buyer’s legal affiliates and/or (ii) a scientific collaborator, provided that each such legal affiliate and/or, scientific collaborator agrees in writing (1) not to transfer such CRISPR-Cas Product or Derived Material to any third party, and (2) to use such CRISPR-Cas Product or Derived Material solely for research as set forth in this limited use label license and not for Commercial Purposes or Development Purposes. If this product is subject to multiple limited use label licenses, the terms of the most restrictive limited use label license shall control.
“Commercial Purpose” means any activity for consideration, including but not limited to: (a) any use, directly or indirectly, in manufacturing and/or production, or quality control ; (b) any use to provide a service, information, or data for consideration; (c) any use for therapeutic, diagnostic or prophylactic purposes; or (d) any sale, resale, leasing, or licensing, whether or not for research purposes.
“Development Purpose” means any clinical activity following IND-enabling pre-clinical toxicological studies or equivalents thereof.
For information on obtaining additional rights to CRISPR-Cas technology for any use not permitted herein, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
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