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Pierce™ Intact Protein Standard Mix
The Thermo Scientific Pierce Intact Protein Standard Mix is a high quality mixture of recombinant proteins for use as aRead more
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| Catalog Number | Quantity |
|---|---|
| A33527 | 5 x 76 μg |
| A33526 | 1 x 76 μg |
Catalog number A33527
Price (EUR)
820,00
Each
-
Quantity:
5 x 76 μg
Price (EUR)
820,00
Each
The Thermo Scientific Pierce Intact Protein Standard Mix is a high quality mixture of recombinant proteins for use as a quality control standard for LC and LC/MS, LC/MS method development, and intact protein mass measurements.
• Verified protein sequences—protein sequences confirmed by MS with the average and monoisotopic masses corresponding to each provided sequence
• Recombinant—proteins expressed in E. coli and B. subtilis; no bovine sources
• Compatible—protein mixture designed for QC over a wide range of molecular weights (10-70 kDa) and m/z distribution (600-1500 m/z)
• Flexible—for use in LC, LC-MS, and direct infusion methods
• Stable—provided in a stable lyophilized format
The Pierce Intact Protein Standard Mix contains a mixture of six highly pure recombinant proteins, including human IGF-1 LR3, human thioredoxin, Streptococcus dysgalactiae Protein G, Bovine Carbonic Anhydrase II, Streptococcus Protein AG (chimeric), and Escherichia coli Exo Klenow, expressed in bacteria. These proteins were selected based on their wide range of molecular weights, m/z distribution, and MS fragmentation patterns. After expression and purification, proteins are optimally mixed and lyophilized for stability and convenience. Each vial contains 76 μg of lyophilized protein mixture and does not contain salts or detergents.
Applications:
• Qualitative LC
• LC/MS standardization and method development
• LC/MS intact protein mass measurement and top-down method development
• Digestion protocol optimization
Protein sequences for Pierce Intact Protein Mix components (FASTA format) ›
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Pierce Peptide Retention Time Calibration Mixture
Pierce 6 Protein Digest, equimolar, LC-MS grade
Pierce HeLa Protein Digest Standard
• Verified protein sequences—protein sequences confirmed by MS with the average and monoisotopic masses corresponding to each provided sequence
• Recombinant—proteins expressed in E. coli and B. subtilis; no bovine sources
• Compatible—protein mixture designed for QC over a wide range of molecular weights (10-70 kDa) and m/z distribution (600-1500 m/z)
• Flexible—for use in LC, LC-MS, and direct infusion methods
• Stable—provided in a stable lyophilized format
The Pierce Intact Protein Standard Mix contains a mixture of six highly pure recombinant proteins, including human IGF-1 LR3, human thioredoxin, Streptococcus dysgalactiae Protein G, Bovine Carbonic Anhydrase II, Streptococcus Protein AG (chimeric), and Escherichia coli Exo Klenow, expressed in bacteria. These proteins were selected based on their wide range of molecular weights, m/z distribution, and MS fragmentation patterns. After expression and purification, proteins are optimally mixed and lyophilized for stability and convenience. Each vial contains 76 μg of lyophilized protein mixture and does not contain salts or detergents.
Applications:
• Qualitative LC
• LC/MS standardization and method development
• LC/MS intact protein mass measurement and top-down method development
• Digestion protocol optimization
Protein sequences for Pierce Intact Protein Mix components (FASTA format) ›
Related products
Pierce Peptide Retention Time Calibration Mixture
Pierce 6 Protein Digest, equimolar, LC-MS grade
Pierce HeLa Protein Digest Standard
For research use only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Mass Spectrometer
Quantity5 x 76 μg
Shipping ConditionWet Ice
Workflow StepCalibration
FormPowder
Product LinePierce™
Product TypeIntact Protein Standard Mix
Unit SizeEach
Contents & Storage
5 vials, store at -5 to -30°C
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Pierce Intact Protein Standard Mix
Representative ESI-MS spectra of Pierce Intact Protein Standard Mix
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3246595Certificate of AnalysisMay 26, 2025A33527
3207339Certificate of AnalysisApr 09, 2025A33527
3200499Certificate of AnalysisMar 31, 2025A33526
2802176Certificate of AnalysisJan 29, 2025A33527
2805271Certificate of AnalysisJan 29, 2025A33526
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Here are our recommendations:
- Pierce Peptide Retention Time Calibration Mixture (Cat. No. 88321) for LC system and gradient optimization
- Pierce BSA Protein Digest, MS grade (Cat. No. 88341), Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342), and Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) for "bottom up" LC-MS method optimization and LC-MS suitability assessment
- Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) (Cat. No. A40010) for assessing the gradient, sensitivity, and dynamic range of the LC-MS system for discovery and targeted peptide quantitative workflows
- Pierce TMT11plex Yeast Digest Standard (Cat. Nos. A40938, A40939) for assessing system performance for TMT-based quantitative workflows
- Pierce Intact Protein Standard Mix (Cat. Nos. A33526, A33527) for "top down" LC-MS method optimization and LC-MS suitability assessment
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.