What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?
Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.
The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
I am unable to download the current software update for the QuantStudio 5 Real-Time PCR System from the Thermo Fisher website. Why?
We recommend trying different browsers (Google Chrome, Firefox, and Microsoft Edge) and/or removing cookies from your browser. If this does not resolve the issue, please contact Technical Support at techsupport@thermofisher.com.
Is the black frame removable from the QuantStudio 3 and 5 Real-Time PCR Systems 96 well, 0.2 ml block?
We do not recommend physically removing the black frame. It is necessary for the plates to fit properly and should stay on the instrument. If it has been accidently removed or lost, please contact ICS to replace the spare part at (800) 9550-6288 options 3-2 or email technicalhardwaresupport@thermofisher.com.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
On which real-time PCR instruments can I use the Quantifiler Duo DNA Quantification Kit?
The Quantifiler Duo DNA Quantification Kit has been optimized and validated for use on the Applied Biosystems 7500 Real-Time PCR System and the Applied Biosystems Human Identification (HID) QuantStudio 5 Real-Time PCR System.