QuantStudio™ 5 Real-Time PCR System for Human Identification, 96-well, 0.2 mL, desktop
QuantStudio™ 5 Real-Time PCR System for Human Identification, 96-well, 0.2 mL, desktop
Applied Biosystems™

QuantStudio™ 5 Real-Time PCR System for Human Identification, 96-well, 0.2 mL, desktop

The Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification with tower computer is the latest addition to ourRead more
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Catalog NumberQuantity
A343221 system
Catalog number A34322
Price (USD)
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1 system
The Applied Biosystems QuantStudio 5 Real-Time PCR System for Human Identification with tower computer is the latest addition to our human identification (HID) real-time PCR family of products. This instrument is designed for both new and experienced users who need a simple, reliable, and affordable real-time PCR system that doesn’t require compromise on performance or quality. When used with our latest advances in quantification chemistry and HID Real-Time PCR Analysis Software, this system offers a sensitive, robust solution for forensic DNA quantification. It also helps prepare laboratories for future quantification technologies. With maximum dye versatility, this HID solution offers accurate, trusted results in a small benchtop footprint.

Features of the QuantStudio 5 Real-Time PCR System for Human Identification:

Streamlined
• Smaller foot print: 27 W x 50 D x 40 H cm, designed to maximize horizontal and vertical bench space
• Front-facing fully motorized block

Interactive
• Touch screen interface enables viewing of amplification plots
• Easy and intuitive interface

Reliable
• Bright light LED median lifetime greater than 5 years
• Less frequent maintenance
• 10 GB on-board memory for storage of 2,000–5,000 runs
• HID-validated system
• 0.2 mL standard 96-well format

Innovative
• Multiplexing capability for up to six dyes, enabling future quantification technology
• Precise temperature control with Peltier-controlled blocks (known as VeriFlex zones)
• USB interface

Real-Time PCR applications
Real-time PCR is a flexible technique that can be used for multiple forensic applications:
• Forensic database
    --Laboratories without direct amplification workflows
• Forensic casework
    --Confirmatory screening of male DNA
    --Sexual assault sample workflow
    --Quality and quantity sample assessment

Specifications
Display TypeTouchscreen
Quantity1 system
Shipping ConditionRoom Temperature
Block FormatNon-interchangeable
Unit SizeEach
Contents & Storage
Desktop Computer

Frequently asked questions (FAQs)

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I am unable to download the current software update for the QuantStudio 5 Real-Time PCR System from the Thermo Fisher website. Why?

We recommend trying different browsers (Google Chrome, Firefox, and Microsoft Edge) and/or removing cookies from your browser. If this does not resolve the issue, please contact Technical Support at techsupport@thermofisher.com.

What is the admin pin for QuantStudio 3 and 5 Real-Time PCR Systems?

If the admin PIN has not been changed, 1234 should work. If it does not work, you can also try 0000.
However, if the admin PIN has been changed and none of the above PINs work, follow the instructions in the QuantStudio 3 and 5 Real-Time PCR Systems manual, page 100.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Is the black frame removable from the QuantStudio 3 and 5 Real-Time PCR Systems 96 well, 0.2 ml block?

We do not recommend physically removing the black frame. It is necessary for the plates to fit properly and should stay on the instrument. If it has been accidently removed or lost, please contact ICS to replace the spare part at (800) 9550-6288 options 3-2 or email technicalhardwaresupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

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