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B-27™ Plus Supplement (50X)
B-27 Plus Supplement is the next-generation of the most cited neuronal cell culture product, B-27 Supplement. It is a serum-freeRead more
| Catalog Number | Quantity |
|---|---|
| A3582801 | 10 mL |
Catalog number A3582801
Price (USD)
187.00
Each
-
Quantity:
10 mL
Delivery information
Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Price (USD)
187.00
Each
B-27 Plus Supplement is the next-generation of the most cited neuronal cell culture product, B-27 Supplement. It is a serum-free supplement that provides the highest rate of in vitro survival of primary rodent and human stem cell-derived neurons when used with Neurobasal Plus Medium. B-27 Plus Supplement features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest rate of neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.
B-27 Plus Supplement, when combined with Neurobasal Plus Medium, offers:
• At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
• Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
• Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
• Accelerates neurite outgrowth and increases the number of synaptic-positive puncta
Available as a 50X 10-mL supplement, B-27 Plus Supplement can seamlessly replace other neuronal cell culture supplements, such as classic B-27 Supplement. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.
B-27 Plus Supplement, when combined with Neurobasal Plus Medium, offers:
• At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
• Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
• Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
• Accelerates neurite outgrowth and increases the number of synaptic-positive puncta
Available as a 50X 10-mL supplement, B-27 Plus Supplement can seamlessly replace other neuronal cell culture supplements, such as classic B-27 Supplement. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Cells
Concentration50 X
Culture TypeMammalian Cell Culture
Product LineB-27™
Quantity10 mL
Serum LevelSerum-Free
Shelf Life12 Months
Shipping ConditionDry Ice
Tested ForEndotoxin, Performance, Sterility
ClassificationSerum-free
FormLiquid
Product TypeCell Culture Supplement
SterilitySterile
Unit SizeEach
Contents & Storage
Store at -5 to -20°C. Protect from light.
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Frequently asked questions (FAQs)
做为一般性的参考方案，我们推荐您使用Gibco B-27添加剂来培养神经干细胞，海马及其他类型的CNS神经元。
-对于来自PNS和CNS中的神经母细胞瘤或有丝分裂后的神经元，可添加Gibco N-2添加剂。
-对于原代胶质细胞（星形胶质细胞）或具有星形胶质细胞表型的肿瘤细胞系（星形胶质细胞和胶质细胞瘤），或少突胶质细胞，可使用Gibco G-5添加剂。
Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
After long-term storage at -20 degrees C, Human Keratinocyte Growth Supplement (HKGS) (Cat. Nos. S0015, S001K) may contain a small amount of precipitate, which is normal. This precipitate is formed from cold insoluble material in the BPE component of the HKGS and will not affect the performance of the product. A similar precipitate may be seen in the BPE component of the HKGS Kit.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We do not recommend thawing B-27 Supplement (Cat. No. A3582801) in a water bath or incubator. We recommend thawing the B-27 Supplement at 4 degrees C overnight.
Note: Remaining B-27 Plus Supplement (50X) may be aliquoted into working volumes and stored at -20 to -5 degrees C. Do not freeze-thaw B-27 Plus Supplement (50X) more than twice. Once thawed do not leave thawed supplement at 4 degrees C for more than two weeks.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.