TrueCut™ Cas9 Protein v2
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TrueCut™ Cas9 Protein v2
Invitrogen™

TrueCut™ Cas9 Protein v2

A next-generation CRISPR Cas9 protein engineered to deliver maximum on-target editing efficiency. TrueCut Cas9 Protein v2 is ideal for applications requiring the highest editing efficiency.
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Catalog NumberQuantityConcentration
A3649610 μg1 μg/μL
A36498100 μg5 μg/μL
A3649725 μg1 μg/μL
A36499500 μg5 μg/μL
Catalog number A36496
Price (USD)
90.65
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92.75
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Quantity:
10 μg
Concentration:
1 μg/μL
Price (USD)
90.65
Online Exclusive
92.75
Save 2.10 (2%)
Each
Add to cart
Ask our AI about this Product

Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency.

Features include:

  • Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in difficult targets than the competition
  • High quality—manufactured under strict ISO 13485 quality standards
  • Validated protocols for a large number of cell types help you achieve success faster

TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.

  • Transfection-ready, using either lipid-mediated transfection reagents or electroporation
  • Eliminates time-consuming cloning steps
  • Available in two concentrations:
    • 1 μg/μL for use in standard editing scenarios
    • 5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
    • For custom sizes or concentrations, please inquire

Options for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:

  • Order pre-designed TrueGuide Synthetic gRNAs for high-efficiency knock out in human or mouse
  • Submit your own design for custom synthesis of CRISPR sgRNA
  • Use our free TrueDesign CRISPR Genome Editor software for easy design and ordering of custom gRNA for precise genome editing
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession NumberNC_002737.2:854751-858857
ComponentsTrueCut Cas9 Protein v2
Expression SystemE. coli
Gene Aliascas5, csn1
Gene ID (Entrez)901176
Gene SymbolSpy_1046
GradeMolecular Biology
Product TypeCas9 Protein v2
Protein FormRecombinant
Protein TagUntagged
Quantity10 μg
Shipping ConditionDry Ice
Validated ApplicationGenome Editing
Concentration1 μg/μL
Product LineTrueCut™
Research CategoryGenome Editing
Unit SizeEach
Contents & Storage
TrueCut Cas9 Protein v2 (1 mg/mL), 10 μg

Store at -5°C to -30°C.
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Frequently asked questions (FAQs)

What is the molecular weight of TrueCut Cas9 Protein v2?

The molecular weight of TrueCut Cas9 Protein v2 is ~163 kD

What is the difference between TrueCut Cas9 Protein (Prototype) and TrueCutCas9 Protein v2?

Both products are the same CRISPR-Cas9 protein, but the TrueCut Cas9 Protein is manufactured with a new production process and formulated to meet the standards for Ancillary Materials for Cell and Tissue-Based Products.

Is there a specific antibody that you recommend to test the delivery of TrueCut Cas9 Protein v2?

Although we do not have a specific antibody recommendation to test the delivery of TrueCut Cas9 Protein v2, we have tested the following antibodies against an older version of Cas9 (Platinum Cas9) using U2OS-Cas9 expression cell lines (generated using Cas9 lentivirus):
- Cas9 Recombinant Polyclonal Antibody (Cat. No. 711061)
- Cas9 Monoclonal Antibody (Cat.No. MA1-201)
- Cas9 Monoclonal Antibody (Cat. No. MA1-202)

Our TrueCut Cas9 Protein v2 and the Platinum Cas9 have similar domains, and the TrueCut Cas9 Protein v2 has better efficiencies. Therefore, these antibodies should work with TrueCut Cas9 Protein v2 as well.

What is the storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

The storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein is as follows:
15 mM Tris-HCl pH 8.0, 150 mM NaCl, 1.0 mM TCEP, 50% glycerol.

Which lipid transfection reagent do you recommend using with TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

We recommend using Lipofectamine CRISPRMAX Transfection Reagent with TrueCut Cas9 Protein v2 or TrueCut HiFi Cas9 Protein.

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