Pierce™ Anti-DYKDDDDK Affinity Resin
Pierce™ Anti-DYKDDDDK Affinity Resin
Thermo Scientific™

Pierce™ Anti-DYKDDDDK Affinity Resin

Thermo Scientific Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins expressedRead more
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Catalog NumberQuantity
A368012 mL
A3680310 mL
A36804100 mL
Catalog number A36801
Price (USD)
581.65
Online Exclusive
648.00
Save 66.35 (10%)
Each
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Quantity:
2 mL
Price (USD)
581.65
Online Exclusive
648.00
Save 66.35 (10%)
Each
Add to cart
Thermo Scientific Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to UltraLink Biosupport. UltraLink Biosupport is a rigid, hydrophilic, highly crosslinked, copolymeric and porous resin with high coupling capacity. The porosity, rigidity, and durability of the resin make it suitable for medium-pressure, fast-flow techniques involving large sample volumes.

For protein purification or IP, resin is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or the C-terminus. Following capture of DYKDDDDK-tagged proteins, non-specifically bound proteins can be washed away before dissociating bound target proteins with elution buffer. The Pierce Anti-DYKDDDDK Affinity Resin is recommended for use in spin purification columns or cartridges for FPLC instruments.

Features include:
Specific—unique base beads and highly specific antibody minimize off-target binding (low non-specific binding)
High purity—optimized bind-wash-elute protocol enables high purity
High yield—special antibody conjugation method enables high yield
Rapid—entire purification protocol typically takes less than 40 mins
Economical—purification protocol allows multiple reuses
Versatile—can be used in spin-, gravity-, or FPLC-based purification and enrichment methods
Scalable—available in 1, 5, and 50 mL settled resin package sizes to allow for smaller scale immunoprecipitations or larger scale purifications

Characteristics of Pierce Anti-DYKDDDDK Affinity Resin:

Composition: anti-DYKDDDDK antibody covalently attached to UltraLink Biosupport, a highly crosslinked rigid support
Particle size: 50–80 μm
pH stability: 1–13
Recommended linear velocity: <150 cm/hour
Operating temperature: 2–30⁰C; do not freeze
Particle concentration:50% slurry in phosphate buffered saline, 0.02% sodium azide, pH 7.5
Binding capacity: ≥3.0 mg DYKDDDDK-tGFP-His protein (∼32 kDa)/mL settled resin
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormLiquid
FormatLiquid
Product LinePierce™
Product TypeAffinity Resin
Quantity2 mL
Shipping ConditionWet Ice
Stationary PhaseAnti-DYKDDDDK monoclonal antibodies
MatrixAffinity Resin
Unit SizeEach
Contents & Storage
1 mL settled resin. 2 mL supplied as a 50% slurry in PBS containing 0.02% sodium azide. Store at 2–8°C.

Frequently asked questions (FAQs)

What is the slurry/suspension percentage for your anti-DYKDDDDK ("FLAG") products?

Anti-DYKDDDDK Magnetic Agarose (Cat. Nos. A36797, A36798, A36799B) is offered as a 25% suspension (1 mL of 25% suspension = 0.25 mL of settled beads). UltraLink-based Anti-DYKDDDDK Affinity Resin (Cat. Nos, A36801, A36803, A36804) is offered as a 50% slurry (1 mL of 50% slurry = 0.5 mL of settled resin).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the binding capacity for your Anti-DYKDDDDH Affinity Resin?

Here is the binding capacity: ≥3.0 mg DYKDDDDK-tGFP-His protein (˜32 kDa)/mL settled resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I use your UltraLink-based Anti-DYKDDDDH ("FLAG") Affinity Resin for FPLC purification?

Yes. The UltraLink-based Anti-DYKDDDDH (“FLAG”) Affinity Resin (Cat. Nos. A36801, A36803, A36804) can be used for FPLC purification.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How do I cleave off the DYKDDDDK ("FLAG") tag after purification?

An enterokinase cleavage site behind the DYKDDDDK (“FLAG”) tag can allow complete removal of the DYKDDDDK (“FLAG”) tag leaving no additional amino acids. We offer EKMax Enterokinase (Cat. Nos. E18001 and E18002) that can be used for this purpose. Subsequently, the EKMax Enterokinase can be removed using EK-Away Resin (Cat. No. R18001), a resin conjugated with soybean trypsin inhibitor, which has high affinity for enterokinase.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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