VetMAX™ MastiType Micro4 Combo Kit
VetMAX™ MastiType Micro4 Combo Kit
Applied Biosystems™

VetMAX™ MastiType Micro4 Combo Kit

This combo kit includes the VetMAX MastiType Micro4 qPCR Kit and the MagMAX CORE Nucleic Acid Purification Kit with MastitisRead more
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Catalog NumberQuantity
A40292100 tests
Catalog number A40292
Price (USD)
-
Quantity:
100 tests
This combo kit includes the VetMAX MastiType Micro4 qPCR Kit and the MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria module (also available separately).

VetMAX MastiType Micro4 Kit
The VetMAX MastiType Micro4 assay enables rapid, accurate detection of the four major contagious mastitis-causing pathogens in bovine milk using real-time PCR amplification of DNA unique to each pathogen in a single reaction. The kit detects these four mastitis-causing pathogens:
Staphylococcus aureus
Streptococcus agalactiae
Mycoplasma bovis
Streptococcus uberis

Learn more about the VetMAX MastiType Micro4 Kit >

MagMAX CORE Nucleic Acid Purification Kit
The MagMAX CORE Nucleic Acid Purification Kit is a highly-effective solution for purifying RNA and DNA from an expansive range of veterinary sample types. The MagMAX CORE sample extraction system is a universal magnetic bead-based separation system designed to meet current and future veterinary testing needs. It is compatible with both automated (using the KingFisher Flex Purification System) and manual purification methods. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing.

Learn more about the MagMAX CORE Nucleic Acid Purification Kit >

MagMAX CORE Mastitis & Panbacteria Module
The MagMAX CORE Mastitis & Panbacteria Module is offered here in combination with the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of all bacteria, especially those important in the detection of mastitis in cattle and from challenging matrices like mastitic milk.

We have included a specially optimized lysis solution, along with an adjusted extraction procedure to improve the recovery of nucleic acid from all bacteria, including gram-positive bacteria.

Features of this combined product include:
Convenience—a complete solution using the same CORE chemistry
Easy—no centrifugation and no aspiration steps
Fast—less than 1 hour for 96 samples
Flexible—appropriate for fresh, frozen, or preserved milk samples, including samples with high somatic cell count

The MagMAX CORE kit and the MagMAX CORE Mastitis & Panbacteria Module help make your lab more efficient through greater convenience, consistent performance, and time and cost savings.
Specifications
For Use With (Equipment)7500 Fast System, 7500 System, QuantStudio™, KingFisher™ Flex
No. of Tests100 tests
Product LineVetMAX™
Product TypePCR Reagents and Detection Kit for Animal Health
Quantity100 tests
Sample TypeOther Samples
Shipping ConditionDry Ice
Detection MethodPrimer-probe
FormFrozen
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
• 1 x 50 mL MagMAX CORE Lysis Solution*
• 1 x 45 mL MagMAX CORE Binding Solution*
• 1 x 60 mL MagMAX CORE Wash Solution 1*
• 1 x 60 mL MagMAX CORE Wash Solution 2*
• 1 x 12 mL MagMAX CORE Elution Buffer*
• 1 x 2.2 mL MagMAX CORE Magnetic Beads*
• 1 x 1.25 mL MagMAX CORE Proteinase K*
• 4 x 1375 μL MagMAX CORE Mastitis PanBacteriaǂ
• 1 x 1.2 mL MastiType Master Mix.ǂ Contains a Hot-Start DNA polymerase in an optimized PCR buffer with magnesium and dNTPs.
• 1x 440 μL MastiType Positive Control.ǂ A positive control for the PCR reaction components.
• 1 × 550 μL MastiType Micro4 Primer Mix.ǂ Includes primers for multiplex PCR amplification of S. aureus, Str. agalactiae, M. bovis, and S. uberis, and an Internal Amplification Control (IAC).

* Store at room temperature.
ǂ Store at -5 to -30°C.

Frequently asked questions (FAQs)

What kind of milk samples can be tested with the VetMAX MastiType kits?

All VetMAX MastiType kits may be used with milk, bulk milk, frozen milk, or preserved milk (e.g., with bronopol).

What qPCR instrument can I use to run the VetMAX MastiType kits?

Either the Applied Biosystems 7500/7500 Fast Real-Time PCR System or the QuantStudio 5 Real-Time PCR System may be used to run the VetMAX MastiType kits.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Which sample prep kit do you recommend upstream of the VetMAX MastiType kits?

We recommend using the MagMAX CORE Nucleic Acid Purification Kit + MagMAX CORE Mastitis & Panbacteria Module (Cat. No. A40289) with the VetMAX MastiType kits.

Which organisms can be detected by each VetMAX MastiType kit?

The VetMax MastiType Micro4 Kit detects Mycoplasma bovis, Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus uberis.

The VetMax MastiType Myco8 Kit detects Mycoplasma spp., Mycoplasma alkalescens, Mycoplasma bovis, Mycoplasma bovigenitalium, Mycoplasma canadense, Mycoplasma californicum, Staphylococcus aureus, and Streptococcus agalactiae.

The VetMax MastiType Multi Kit detects Staphylococcus aureus, Staphylococcus spp. (including all major coagulase-negative staphylococci), Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Escherichia coli, Enterococcus spp. (incl. E. faecalis and E. faecium), Klebsiella oxytoca and/or Klebsiella pneumoniae, Serratia marcescens, Corynebacterium bovis, Trueperella pyogenes and/or Peptoniphilus indolicus, Staphylococcal beta-lactamase gene (penicillin-resistance gene), Mycoplasma bovis, Mycoplasma spp., Yeast, and Prototheca spp.

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