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Fetal Bovine Serum, Tet system approved, US origin
Fetal Bovine Serum, Tet system approved, US origin
Gibco™

Fetal Bovine Serum, Tet system approved, US origin

Tet system-approved FBS, also known as tetracycline-free FBS, is specifically formulated for cell culture applications utilizing tetracycline and tetracycline derivative-regulated gene expression systems.
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Catalog NumberQuantityFormat
A473610150 mLOne Shot
A4736201500 mLBottle
Catalog number A4736101
Price (USD)
138.65
Online Exclusive
155.00
Save 16.35 (11%)
Each
Add to cart
Quantity:
50 mL
Format:
One Shot
Price (USD)
138.65
Online Exclusive
155.00
Save 16.35 (11%)
Each
Add to cart

Gibco Fetal bovine sera include a broad range of specialty sera, earning the trust of researchers with consistent quality and award-winning support that helps meet your unique research requirements. Tet system-approved FBS is designed to ensure the absence of detectable tetracycline and its derivatives that may interfere with accurate regulation of gene expression, enhancing the accuracy and reliability of research outcomes.

Gibco Tet system-approved FBS is formulated for cell culture applications using tetracycline- and tetracycline derivative-regulated gene expression systems. Cell-based assays that detect the presence of tetracycline and tetracycline analogs are used to select FBS that delivers the highest range of induction.

  • Designed to ensure the absence of detectable tetracycline and its derivatives that may interfere with accurate regulation of gene expression
  • Functional testing provides confidence that unintended tetracyline gene expression will not result
  • Sera Category: Specialty
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
Cell TypeUse for a broad range of cell types, especially sensitive cell lines
ClassificationPremium
Endotoxin Concentration<5 EU/mL
GradePremium
Green FeaturesSustainable packaging
Hemoglobin Concentration<15 mg/dL
Purity or Quality GradePremium
Shelf Life3 Years
Shipping ConditionFrozen/Dry Ice
Specialty TypeTet-System Approved
SpeciesCattle/Bovine
Tested ForMeets USP/EP guidelines with up to 91 harmonized quality specification tests
AgeFetal
Country of OriginUnited States
FormatOne Shot
Product TypeFetal Bovine Serum
Quantity50 mL
SterilitySterile
Sterilization MethodTriple-filtered, 0.1 μm
Unit SizeEach
Contents & Storage
Store at <-10°C.

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Why do you use a cell-based assay instead of HPLC to determine the tetracycline concentration/presence in Fetal Bovine Serum, Tet system approved?

While competitors may use HPLC or other assays to determine the presence of tetracycline, we developed a cell-based assay because we wanted to verify that the tetracycline present, if any, in this product would not be enough to alter the experiment. The only way to confirm this is to actually demonstrate the efficacy of the serum in a dish with cells (cell-based assay).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How is Fetal Bovine Serum, Tet system approved verified to be tetracycline-free?

Fetal Bovine Serum, Tet system approved is not tetracycline-free, and no serum can be absolutely confirmed to be tetracycline-free without altering the performance of the product. We developed a cell-based assay to confirm that tetracycline, if present, is not in high enough concentrations to alter Tet experiments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer Fetal Bovine Serum, Tet system approved that has already been heat inactivated?

Sorry, we do not offer Fetal Bovine Serum, Tet system approved that has already been heat inactivated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it normal to have precipitation in Fetal Bovine Serum, Tet system approved?

Since our sera are not pre-aged before filtration, turbidity or flocculent debris may develop upon thawing or storage. This flocculence is a normal characteristic of the serum and does not adversely affect performance of the serum.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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