MagMAX™ Wastewater Ultra Nucleic Acid Isolation Kit with Virus Enrichment
MagMAX™ Wastewater Ultra Nucleic Acid Isolation Kit with Virus Enrichment
Applied Biosystems™

MagMAX™ Wastewater Ultra Nucleic Acid Isolation Kit with Virus Enrichment

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit with Virus Enrichment enables efficient, high-throughput virus enrichment and nucleic acid extractionRead more
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Catalog NumberQuantity
A52610100 Preps
Catalog number A52610
Price (USD)
1,258.65
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1,316.00
Save 57.35 (4%)
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Quantity:
100 Preps
Price (USD)
1,258.65
Online Exclusive
1,316.00
Save 57.35 (4%)
Each
Add to cart
The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit with Virus Enrichment enables efficient, high-throughput virus enrichment and nucleic acid extraction from wastewater samples. The kit utilizes Dynabeads and MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid compatible with a broad range of applications, including real-time PCR and next-generation sequencing.

Features of the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit include:
• Automation-ready concentration and extraction protocols designed for KingFisher Flex, KingFisher APEX, and KingFisher Duo systems
• Fast procedure allows for virus enrichment and nucleic acid extraction for 24 wastewater samples in 75 minutes
• Dynabeads wastewater virus enrichment is used to concentrate wastewater before extraction
• Elution volumes ranging from 50 to 100 μL

Wastewater and sewage surveillance is a useful tool for monitoring the regional spread of viral and bacterial pathogens. Surveillance at different levels of operation, such as a university dormitory or an entire city, has been shown to be a reliable way to detect the SARS-CoV-2 virus before it becomes widespread in a population. Wastewater surveillance can be used to monitor other viral targets such as influenza and norovirus. However, wastewater and sewage samples are some of the most challenging when it comes to extraction of RNA and DNA, until now.

Automated virus enrichment
Up to 10 mL of wastewater can be processed, with virus concentration followed by extraction. When the virus enrichment and extraction process is performed using a KingFisher Flex or APEX system, 24 samples can be processed in just 75 minutes. When the protocol is completed, pure RNA and DNA is concentrated in 50–100 μL of elution buffer. If other concentration methods, such as ultracentrifugation or precipitation are desired, the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit is available for 200 μL to 500 mL input volumes and includes only extraction reagents, enabling users to use a concentration method of choice, filtration, or non-concentrated wastewater samples.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid. Beads bind the DNA and RNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples.

The Dynabeads MyOne Silane contained in the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads MyOne Silane ideal for the recovery of RNA and DNA. Once captured, these nucleic acids can then be eluted in of 50–100 μL of elution buffer for use in downstream applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume100 to 500 μL
Final Product TypeRNA and DNA
For Use With (Application)RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
For Use With (Equipment)KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
Quantity100 Preps
Sample TypeWastewater
Shipping ConditionRoom Temperature
Starting Material Amount15 mL
Test TimeUp to 45 min. when using automated protocol
Isolation TechnologyMagnetic Bead
Unit SizeEach
Contents & Storage
• 10 mL Dynabeads Wastewater Virus Enrichment; 4°C
• 80 mL Lysis Buffer; room temperature
• 50 mL Binding Solution; room temperature
• 200 mL Wash Buffer; room temperature
• 25 mL Elution Solution; room temperature
• 4 mL Proteinase K; room temperature
• 2 mL DNA/RNA Binding Beads; room temperature

Frequently asked questions (FAQs)

Is the purified viral nucleic acid that is obtained using MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment completely inhibitor-free?

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment allows elimination of the vast majority of diverse inhibitors of enzymatic reactions, even from the most diluted and challenging samples such as wastewater. In very rare instances, if some inhibitors still remain within the purified nucleic acid, the sample can be diluted 10-20X prior to downstream PCR or other applications.

What starting volume of wastewater can be used with the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment?

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment is compatible with starting volumes of 1-10 mL. If concentration is not needed, or if wastewater has been concentrated using ultracentrifugation or precipitation, use the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit (Cat. No. A52606).

How long is the protocol for the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment?

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment protocol takes 90 mins to concentrate virus and isolate nucleic acid.

Is the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment automation-compatible?

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment is compatible with the KingFisher Flex, APEX, and Duo instruments, and can also be used for manual processing in 24- and 96-well plates.

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