Residual Protein TrypLE™ ELISA Kit
Residual Protein TrypLE™ ELISA Kit
Gibco™

Residual Protein TrypLE™ ELISA Kit

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLERead more
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Catalog NumberQuantity
A653310196 Tests kit
A65331025 kits, 480 tests
A653310310 kits, 960 tests
Catalog number A6533101
Price (USD)
542.00
Each
-
Add to cart
Quantity:
96 Tests kit
Price (USD)
542.00
Each
Add to cart

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme. The kit is compatible with all forms of TrypLE enzyme, including TrypLE Select, TrypLE Express, and CTS TrypLE Select enzymes.

TrypLE enzyme is an animal-origin-free recombinant alternative to porcine trypsin. It is used for the dissociation of attachment-dependent cell lines. TrypLE enzyme dissociates cells cultured both in serum-free and serum-supplemented systems. Due to the unique biological sequence of TrypLE enzyme, traditional trypsin ELISA kits are ineffective at binding and detecting residual levels of TrypLE enzyme.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Assay Range0.78 to 50 ng/mL
Assay Sensitivity0.032 ng/mL
ConjugateHorseradish Peroxidase (HRP)
For Use With (Equipment)Colorimetric Microplate Reader
FormatWash steps required
Interassay CV6.20%
Intraassay CV7.20%
Label or DyeHorseradish Peroxidase (HRP)
Protein AliasesTrypLE, R-Protease
Quantity96 Tests kit
Shipping ConditionWet Ice
TargetTrypLE (R-Protease) concentration
Total Assay Time1.5 hr.
Immunoassay Kit FormatSandwich ELISA Kit
Unit SizeEach
Contents & Storage
• Pre-coated 96-well plate
• Standard assay buffer concentrate
• HRP-conjugated detection antibody
• Wash buffer concentrate
• Chromogen stop solution
• Adhesive plate covers

Store at 2–8°C.

Frequently asked questions (FAQs)

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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