Tetraethylene Glycol Monooctyl Ether (C8E4) is a non-ionic surfactant that possesses unique properties making it suitable for solubilization of sensitive biomolecules without significant denaturation or disruption of their structure and function. It has good solubility in both aqueous and organic solvents, allowing for versatile applications. C8E4 can be used as a detergent in native MS to solubilize and stabilize biomolecules, particularly membrane proteins or protein complexes, in aqueous solutions. It helps maintain the native conformation of these biomolecules and facilitates their transfer into the gas phase during the MS analysis.

Features of C8E4 include:
• Non-ionic detergent
• Soluble in both water and organic solvents
• Enhanced stability of solubilized biomolecules, including membrane proteins
• High-purity compound
• Native mass spec compatible

Applications
C8E4 is versatile and can be used in various applications in addition to native MS, including protein purification, crystallization, and structural biology studies. It is compatible with a wide range of biomolecules and can be employed for both soluble and membrane protein research.

Usage
Optimizing the concentration of C8E4 is crucial to ensure effective solubilization and stabilization of the biomolecules while minimizing detergent interference in the mass spectrometry analysis. Additionally, the specific conditions and parameters of the native MS experiment, such as the choice of ionization source and mass spectrometer settings, should be carefully considered and optimized for each specific application.

Properties
• CMC: (0.1% NaCl) ~8 mM (0.25%)
• Aggregation number: (H₂O) ~82
• Purity: ≥99% by HPLC analysis
• pH: 5–8 (5% solution in water)