Verso 1-step RT-qPCR Mix, low ROX
Product Image
Thermo Scientific™

Verso 1-step RT-qPCR Mix, low ROX

The Thermo Scientific Verso 1-step RT-qPCR Low ROX Mix Kit for probe chemistry offers a quick and simple way toRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberNo. of Reactions
AB4102A
also known as AB-4102/A
200 Reactions
AB4102C
also known as AB-4102/C
400 Reactions
Catalog number AB4102A
also known as AB-4102/A
Price (USD)
674.00
Each
Add to cart
No. of Reactions:
200 Reactions
Price (USD)
674.00
Each
Add to cart
Ask our AI about this Product
The Thermo Scientific Verso 1-step RT-qPCR Low ROX Mix Kit for probe chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

Features of the Verso 1-step RT-qPCR Low ROX Mix Kit:

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction set up

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.

Applications

• Gene expression analysis
• RNAi validation
• Microarray validation
• Pathogen detection
• Genetic testing
• Disease research

Related Products
Verso 1-step RT-qPCR Mix, low ROX
Verso 1-step RT-qPCR Mix, ROX
Verso 1-step RT-qPCR Kit, with separate ROX vial
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
FormatTube
No. of Reactions200 Reactions
Product LineVerso
Product Type1-Step RT-qPCR Mix
Quantity200 reactions
Sample TypeRNA
Sufficient For200 Reactions
Detection MethodPrimer-probe
For Use With (Application)Gene Expression, Pathogen Detection
GC-Rich PCR PerformanceHigh
PCR Method1-step RT-qPCR
Unit SizeEach
Contents & Storage
Contains:
•1 x 50 μL Verso Enzyme Mix
•1 x 250μL RT enhancer
•2 x 1.25 mL 2X 1-Step qPCR Low ROX Mix

Store at -20°C
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

What is the RT Enhancer that is included in all Verso kits?

The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.

Do I need to treat my RNA with DNase I before running RT-qPCR with Verso cDNA Synthesis Kit?

No, the Verso cDNA Syntheis Kits include RT Enhancer that, when included in the cDNA synthesis reaction, removes contaminating DNA eliminating the need for DNase I treatment.

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link

1x1 image pixel for data collection