The Ambion™ MAXIscript™ SP6/T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protectionRead more
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Catalog Number
Product Type
Quantity
AM1320
SP6/T7 In Vitro Transcription kit
30 reactions
AM1322
SP6/T7 In Vitro Transcription kit
100 Reactions
Catalog number AM1320
Price (USD)/ 30 reactions
239.00
-
Add to cart
Product Type:
SP6/T7 In Vitro Transcription kit
Quantity:
30 reactions
Price (USD)/ 30 reactions
239.00
Add to cart
MAXIscript™ SP6/T7 Transcription Kit
Catalog numberAM1320
Price (USD)/ 30 reactions
239.00
-
Add to cart
The Ambion™ MAXIscript™ SP6/T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 50 reactions of each enzyme.
• High yields of high specific activity, full-length RNA probes in just 10 minutes • Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization • Includes TURBO DNase™ • All kits contain quality-tested DNase/RNase-free reagents
The MAXIscript™ In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/μg in just 10 minutes. MAXIscript™ Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript™ Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript™ Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 μg/20 μl reaction) of up to 2 kb.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeSynthetic mRNA, mRNA
FormatKit
Sample TypemRNA, Synthetic mRNA
For Use With (Application)In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions30 Reactions
Product LineAmbion™, MAXIscript™
Product TypeSP6/T7 In Vitro Transcription kit
Quantity30 reactions
PromoterT7, SP6
Shipping ConditionDry Ice
Yield2-6 μg
Starting MaterialPCR Products, Linearized DNA plasmid
Unit Size30 reactions
Contents & Storage
SP6 Enzyme Mix, T7 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.