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DNA-free™ DNA Removal Kit
DNA-free™ DNase treatment and removal reagents are designed for the removal of contaminating DNA from RNA samples and for theRead more
| Catalog Number | Quantity |
|---|---|
| AM1906 | 50 reactions |
Catalog number AM1906
Price (EUR)
188,65
Online Exclusive
209,00Save 20,35 (10%)
Each
-
Quantity:
50 reactions
Price (EUR)
188,65
Online Exclusive
209,00Save 20,35 (10%)
Each
DNA-free™ DNase treatment and removal reagents are designed for the removal of contaminating DNA from RNA samples and for the removal of DNase after treatment. Features of this reagent set include:
• Safely eliminate DNA contamination from RNA samples
• No organic extraction or heat inactivation required
• Includes novel reagent to remove DNase
• Recombinant DNase I is certified RNase-free
Inactivation and removal of DNase
DNA-free™ reagents effectively remove DNase and divalent cations from the reaction mixture. The DNase/cation removal step takes only three minutes. No organic extraction, EDTA addition, or heat inactivation is required. The DNA-free™ Kit comes complete with RNase-free DNase I, an optimized 10X Reaction Buffer, and a novel DNase Removal Reagent.
Accessory product
TURBO DNA-free™ Kit (Cat. No. AM1907) is similar to the DNA-free™ Kit but includes TURBO™ DNase, an engineered hyperactive DNase that exhibits up to 350% greater catalytic efficiency than wild type DNase. The enzyme also has a 6-fold lower Km for DNA, thus enabling effective removal of trace quantities of DNA contamination.
• Safely eliminate DNA contamination from RNA samples
• No organic extraction or heat inactivation required
• Includes novel reagent to remove DNase
• Recombinant DNase I is certified RNase-free
Inactivation and removal of DNase
DNA-free™ reagents effectively remove DNase and divalent cations from the reaction mixture. The DNase/cation removal step takes only three minutes. No organic extraction, EDTA addition, or heat inactivation is required. The DNA-free™ Kit comes complete with RNase-free DNase I, an optimized 10X Reaction Buffer, and a novel DNase Removal Reagent.
Accessory product
TURBO DNA-free™ Kit (Cat. No. AM1907) is similar to the DNA-free™ Kit but includes TURBO™ DNase, an engineered hyperactive DNase that exhibits up to 350% greater catalytic efficiency than wild type DNase. The enzyme also has a 6-fold lower Km for DNA, thus enabling effective removal of trace quantities of DNA contamination.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible Buffer10X Reaction Buffer
Product TypeDNA Removal Kit
Quantity50 reactions
Shipping ConditionDry Ice
EnzymeDNase
Product LineAmbion™, DNA-free™
Unit SizeEach
Contents & Storage
Store rDNase1 , 10X DNase 1 Buffer and DNase Inactivation Reagent at -20°C. Store Nuclease–free Water at room temperature.
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Figures
Removal of divalent cations by DNase Inactivation Reagent.
Inactivation of DNase I by DNase Inactivation Reagent.
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Lot #Certificate TypeDateCatalog Number(s)
3265597Certificate of AnalysisJun 20, 2025AM1906
3265588Certificate of AnalysisJun 20, 2025AM1906
3247790Certificate of AnalysisMay 27, 2025AM1906
3219643Certificate of AnalysisApr 16, 2025AM1906
3198698Certificate of AnalysisMar 27, 2025AM1906
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Safety Data Sheets
SDSProduct Information
Frequently asked questions (FAQs)
Add of 1 µL of 25 mM EDTA solution to the reaction mixture in 10 µL reaction with 1 unit DNase I, Amplification Grade (or 1:1 molar ratio of Mg++ ions:EDTA) to chelate the Mg++ ions in the DNase I buffer. Heat for 10 min at 65 degrees C.
Note: It is vital that the EDTA be at least at 2 mM prior to heat-inactivation to avoid Mg-dependent RNA hydrolysis.
DNA-free and Turbo-free versions of DNase I can be inactivated with included DNase Inactivation Reagent.
Most of our DNase I products are guaranteed free of RNase activity. However, please note that Cat. No. 18047-019 is not tested for RNAse and is recommended primarily for protein applications. The other products are suitable for removing DNA from both RNA and protein preparations, for nick translating DNA, and for generating random fragments of DNA. For more demanding RT-PCR applications, we recommend using DNAse I, Amplification Grade.
We recommend that you include DNase treatment of all samples as a good practice, despite the fact that the majority of primers span across an intron and do not amplify (or in some cases, minimally amplify) contaminating genomic DNA. For more information see here.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center