Inquire about OEM or Commercial Supply version of this product here.
Invitrogen™
Ambion™ RNase III
Ambion™ Escherichia coli ribonuclease III (RNase III; EC 3.1.24) is a double-stranded RNA (dsRNA) specific endoribonuclease. In E. coli, RNaseRead more
Have Questions?
Catalog number AM2290
Price (USD)/ 250 units
363.65
Online exclusive
368.00
Save 4.35 (1%)
-
Add to cart
Request bulk or custom format
Price (USD)/ 250 units
363.65
Online exclusive
368.00
Save 4.35 (1%)
Add to cart
Ambion™ RNase III
Catalog numberAM2290
Price (USD)/ 250 units
363.65
Online exclusive
368.00
Save 4.35 (1%)
-
Add to cart
Ambion™ Escherichia coli ribonuclease III (RNase III; EC 3.1.24) is a double-stranded RNA (dsRNA) specific endoribonuclease. In E. coli, RNase III cleaves ribosomal RNA (rRNA) precursors during maturation of rRNA. The enzyme cleaves dsRNA into 12–15 bp dsRNA fragments with 2 to 3 nucleotide 3' overhangs, and 5' phosphate and 3' hydroxyl termini. The termini and overhangs of RNase III cleavage products are thus the same as those produced by Dicer in the eukaryotic RNAi pathway. Transfection of RNase III cleavage products can be used to induce RNAi in mammalian cells (patent pending).
Unit Definition: One unit is defined as the amount of enzyme catalyzing the cleavage of 1 μg of 500 bp dsRNA substrate to approximately 12–30 bp fragments in 60 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Shipping ConditionDry Ice
EnzymeRNase
Compatible Buffer10X Reaction Buffer
Quantity250 units
Product TypeRNase III
Product LineAmbion™
Unit Size250 units
Contents & Storage
RNase III and 10X Reaction Buffer should be stored at –20°C.