Ammonium Acetate (5 M), RNase-free
Ammonium Acetate (5 M), RNase-free
Invitrogen™

Ammonium Acetate (5 M), RNase-free

Ambion Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 500 mL. The solution isRead more
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Catalog NumberQuantity
AM9070G100 mL
AM9071500 mL
Catalog number AM9070G
Price (USD)
60.65
Online Exclusive
63.75
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Quantity:
100 mL
Recurring order eligible. Learn more »
Price (USD)
60.65
Online Exclusive
63.75
Save 3.10 (5%)
Each
Add to cart
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Ambion Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 500 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion's nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialAmmonium Acetate
Packaging TypeBottle
Product LineAmbion™
PurityMolecular Biology Grade
Quantity100 mL
Shipping ConditionRoom Temperature
Concentration5 M
FormLiquid
Unit SizeEach
Contents & Storage
Store at room temperature.
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What salts can be used to precipitate total RNA? What are the advantages of each? Do they require ethanol?
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3255436Certificate of AnalysisJun 06, 2025AM9070G
3255432Certificate of AnalysisJun 06, 2025AM9070G
3255431Certificate of AnalysisJun 06, 2025AM9070G
3197955Certificate of AnalysisMar 26, 2025AM9070G
3162616Certificate of AnalysisFeb 13, 2025AM9071
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Safety Data Sheets

Frequently asked questions (FAQs)

The three salts that can be used are:

- Guanidine thiocyanate, which requires ethanol. Guanidine thiocyanate is a common agent used for isolating RNA and we recommend it especially for tissues high in ribonuclease activity, such as the pancreas or spleen.
- Ammonium acetate, which requires ethanol. Ammonium acetate is useful when reducing coprecipitation of unwanted dNTPs and oligosaccharides. However, it should not be used when the nucleic acid will be phosphorylated using T4 polynucleotide kinase, since this enzyme is inhibited by ammonium ions.
- Lithium chloride, which does not require ethanol. LiCl is very effective in precipitating RNA but does not efficiently precipitate protein or DNA. It also does not precipitate unincorporated nucleotides.

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