Countess™ 3 Automated Cell Counter
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Experience nuclei counting with fluorescent-mode counting algorithms, simplified workflows, built-in dilution calculators and reports using the latest version of Countess software with optional 21 CFR Part 11 compliance. Download now!
Countess™ 3 Automated Cell Counter
Invitrogen™

Countess™ 3 Automated Cell Counter

Experience rapid cell counting with the Countess 3 Automated Cell Counter. Equipped with advanced optics, automated focus and lighting, and AI-based image analysis software, the Countess 3 brightfield cell counter enables automated cell counting and measurement of cell viability.
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Catalog NumberTypeWarranty
AMQAX2000Promo ImageAutomated Cell Counter1-year Standard
A49862Promo ImageAutomated Cell Counter3-year Rapid Exchange (REX) Extended Warranty (1 year standard warranty + 2 years Rapid Exchange, 3 years total exchange entitlement)
A49865Promo ImageAutomated Cell Counter Starter Package1-year Standard
A49891Promo ImageAutomated Cell Counter3-year AB Repair Center (ABRC) Extended Warranty (1 year standard warranty + 2 years extended repair coverage)
A50298Automated Cell Counter Starter Package3-year Rapid Exchange (REX) Extended Warranty (1 year standard warranty + 2 years Rapid Exchange, 3 years total exchange entitlement)
A50302Promo ImageAutomated Cell Counter Starter Package3-year AB Repair Center (ABRC) Extended Warranty (1 year standard warranty + 2 years extended repair coverage)
Catalog number AMQAX2000
Price (USD)
4,709.65
Online Exclusive
5,080.00
Save 370.35 (7%)
Each
Add to cart
Type:
Automated Cell Counter
Warranty:
1-year Standard
Price (USD)
4,709.65
Online Exclusive
5,080.00
Save 370.35 (7%)
Each
Add to cart
The Countess 3 Automated Cell Counter is a brightfield cell counter equipped with an advanced machine-learning algorithm, ruggedized optics, fully-automated focus and lighting, and image analysis software. This benchtop cell counting system is designed for rapid automated cell counting and viability measurement of primary and immortalized cell samples to prepare cells for downstream analysis and for routine cell culture passaging.

Features of the Countess 3 Automated Cell Counter include:

  • Accuracy—highly accurate machine-learning algorithm applied to automated cell counting, eliminates the subjectivity of manual cell counting and user-to-user variability
  • Time savings—counts live and dead cells, measures viability, calculates percent cell aggregates, and reports average cell size with a single touch in about 20 seconds
  • Convenience—requires no cleaning, routine maintenance, or calibration; flush-mounted high-resolution capacitive touchscreen and simple user interface provide quick startup, require minimal training, and offer automatic saving of data to USB or cloud
  • Flexibility—compatible with both disposable and reusable cell counting chamber slides

The Countess 3 Automated Cell Counter works in two simple steps:

  1. Mix 10 μL of sample with 10 μL of trypan blue and pipet into a disposable or reusable Countess chamber slide.
  2. Insert the slide into the instrument. Rapid Capture and Auto-Save modes allow automated cell counting with a single touch. Focus, lighting, and file saving are executed automatically.

Obtain accurate cell counts

The Countess 3 Automated Cell Counter uses trypan blue staining combined with auto-focus and lighting driven by a machine-learning image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 x 104 to 1 x 107 cells/mL (the optimal range is 1 x 105 to 4 x 106 cells/mL), which is broader than that of a hemocytometer.

Transfer data and images to your PC or cloud

Using the USB port on the Countess 3 Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. For your convenience, a USB drive is supplied with the Countess 3 instrument, and a WiFi dongle is available.

Improve downstream single-cell analysis

Minimizing cell aggregates is crucial for RNA sequencing applications to ensure that individual cells are partitioned and barcoded during the single-cell library preparation process. For optimal single-cell data output, the percent cell aggregates on the Countess 3 Automated Cell Counter can be used to confirm that the starting sample has <5% aggregates before performing downstream single-cell analysis.

The Countess 3 Automated Cell Counter can be purchased as the instrument (Cat. No. AMQAX2000) or a variety of packages bundled with additional accessories and extended warranties such as the Countess 3 Starter Package (Cat. No. A49865), which includes:

  • Countess 3 Automated Cell Counter
  • Reusable slide
  • 500 disposable slides with trypan blue

Extended warranty instrument and starter packages

The Countess 3 Automated Cell Counter comes with a standard 1-year warranty that covers all costs for shipping, parts, and labor for repairs. Service plans can be bundled with new instrument purchases in an extended warranty package that extends the standard warranty by 2 years for a total of 3. Additional years of coverage can also be purchased to extend the standard 1-year or extended 3-year warranty. The Countess 3 instrument and starter packages are offered with two different types of bundled extended warranty packages:

  • 3-year Rapid Exchange (REX) Extended Warranty (1 year standard warranty + 2 years Rapid Exchange, 3 years total exchange entitlement)
    • Replacement shipped out in 1 business day for labs with critical timelines
    • Available with the instrument only (Cat. No. A49862) and the Countess 3 Starter Package (Cat. No. A50298)
  • 3-year AB Repair Center (ABRC) Extended Warranty (1 year standard warranty + 2 years extended repair coverage)
    • Instrument repaired and returned in ~3 weeks for regulated labs required to maintain asset tagging consistency
    • Available with the instrument only (Cat. No. A49891) and the Countess 3 Starter Package (Cat. No. A50302)

21 CFR Part 11 Software Module

The Countess 3 21 CFR Part 11 Software Module is for use with the Countess 3 and Countess 3 FL automated cell counters and supports compliance with 21 CFR Part 11 FDA guidelines for security, auditing, and electronic (SAE) signatures using an SAE administration console. The 21 CFR Part 11 software can be purchased separately (Cat. No. A51025).

For Research Use Only
Specifications
Certifications/ComplianceRoHS
Detection MethodBrightfield
Dimensions9 x 9 x 5.5 in. (228.6 x 228.6 x 139.7 mm)
DisplayLCD (1280 x 800 pixels)
Electrical Requirements12 VDC, 3 A
For Use With (Application)Cell Counting and Viability Measurement
For Use With (Equipment)EVOS Microscopes, Countess Chamber Slides, and Reusable Slide
Frequency50/60 Hz
Holds2 samples per slide
Magnification2.5X
MemoryOnboard, USB Drive
Optics1 Channel (Brightfield)
Quantity1 instrument
Shipping ConditionAmbient
Voltage100/240 V
Warranty1-year Standard
Weight (English)8 lb.
Weight (Metric)3.6 kg
Camera5 MP
Product LineCountess
TypeAutomated Cell Counter
Unit SizeEach
Contents & Storage
  • Countess™ 3 Automated Cell Counter, 1 each
  • Power cord with 4 adapters (for US/Canada/Taiwan/Japan, Europe, or UK), 1 each
  • Countess™ Cell Counting Chamber Slides, 50 slides/box
  • Countess™ 3 Disposable Slide Holder, 1 each
  • Countess™ 3 Reusable Slide Holder, 1 each
  • Countess™ 3 USB Drive, 1 each
  • Countess™ 3 Automated Cell Counter Quick Reference Card, 1 each

Store at room temperature.

Frequently asked questions (FAQs)

Why am I getting a much higher bead count on the Countess 3 Standard Slide (Cat. No. A51876)?

When counting cells that have been mixed with Trypan Blue, the Countess accounts for this 1:1 dilution and multiplies the count by 2.
When using the Countess 3 Standard Slide, the Trypan Blue correction box needs to be unchecked, since a 1:1 dilution was not made with Trypan Blue.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What cell counter do you offer that can count live and dead cells, measure cell viability, cell size, has high-resolution touch screen, and comes with reusable slides?

We suggest the Countess 3 and 3 FL Automated Cell Counters with starter packages; please see the product pages linked below:

Countess 3 FL Automated Cell Counter Starter Package + ABRC Extended Warranty (Cat. No. A50303)

Countess 3 FL Automated Cell Counter Starter Package + REX Extended Warranty (Cat. No. A50299)

Countess 3 FL Automated Cell Counter Starter Package (Cat. No. A49866)

Countess 3 Automated Cell Counter Starter Package + ABRC Extended Warranty (Cat. No. A50302)

Countess 3 Automated Cell Counter Starter Package + REX Extended Warranty (Cat. No. A50298)

Countess 3 Automated Cell Counter Starter Package (Cat. No A49865)

For more information about out Countess 3 Automated Cell Counters See the brochure linked below:

linkCountess 3 Automated Cell Counters: A counter that thinks like a cell biologist

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are there any recommended parameter settings on the Countess 3/3FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000) for specific cell lines?

No. The Countess 3/3 FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000) parameter settings, especially brightness, can vary between instruments due to slight differences in lamp output and other factors.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What can we do with the FCS file generated by the Countess 3/3 FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000)?

The Countess 3/3FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000) FCS files can be uploaded to most flow cytometry software that can accept FCS files, such as FlowJo or the Attune NxT software. Using the flow cytometry software, you may see the distribution of all cells and adjust/fine-tune parameter settings for only the cells of interest or analyze outliers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can we use any EVOS Light Cube with the Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000)?

With the exception of EVOS Light Cube, White (Cat no. AMEP4672) any of the EVOS Light Cubes for fluorescence detection can be used on the Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Documents & Downloads

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