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Alomone Labs, LTD
Robo3 (extracellular) Polyclonal Antibody
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Application
FIGURE: 1 / 4
Robo3 (extracellular) Antibody (ANR-183-25UL) in IHC (F)
Expression of Robo3 in rat cerebellum. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Robo3 (extracellular) Antibody (#ANR-183) (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Robo3 immunoreactivity (green) appears in cerebellar neurons (arrows). B. Pre-incubation of the antibody with Robo3 (extracellular) Blocking Peptide (BLP-NR183), suppressed staining. Cell ... View More
Product Details
ANR-183-25UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide CHQKIDDLFSGK, corresponding to amino acid residues 190-201 of human CD81. Extracellular, 2nd loop.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunogen sequence: CHQKIDDLFSGK
Reconstitution: 25 uL, 50 uL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ21044; retinoblastoma inhibiting gene 1; Retinoblastoma-inhibiting gene 1 protein; Rig-1; Roundabout guidance receptor 3; Roundabout homolog 3; roundabout, axon guidance receptor, homolog 3; Roundabout-like protein 3
Gene Aliases: HGPPS; HGPS; RBIG1; Rig-1; RIG1; ROBO3; Robo3a; Robo3b
UniProt ID: (Human) Q96MS0, (Mouse) Q9Z2I4
Entrez Gene ID: (Human) 64221, (Rat) 315564, (Mouse) 19649
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