20X Bolt™ MES SDS Running Buffer

Certificates

SDS

Invitrogen™

20X Bolt™ MES SDS Running Buffer

Bolt MES SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris gels. It is recommended for separatingRead more

Change view

Catalog Number	Quantity
B000202	5 L
B0002	500 mL

2 Options

Catalog number B000202

Price (USD)

680.65

Online Exclusive

704.00

Save 23.35 (3%)

Each

In stock

Add to cart

Quantity:

5 L

Price (USD)

680.65

Online Exclusive

704.00

Save 23.35 (3%)

Each

Add to cart

Bolt MES SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

WARNING: Cancer – www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Name or MaterialRunning Buffer

FormulationSodiumdodecyl Sulphate 151-21-3 ( 1-5 )

Recommended StorageMES SDS Running Buffer (20X).

Store at 4°C to 25°C.

Shelf-life is 6 months, when stored properly.

Concentration20X

For Use With (Application)Protein electrophoresis & western blotting

For Use With (Equipment)Bolt

Physical FormLiquid

Product LineBolt

Quantity5 L

Unit SizeEach

Are Bolt gels compatible with other transfer devices?

Are Bolt gels compatible with transfer devices from other suppliers?

Is there a difference in composition between the NuPAGE MES SDS Running Buffer (20X) and the 20X Bolt MES SDS Running Buffer?

Which power supplies are compatible with the Mini Gel Tank?

This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

View all 20X Bolt™ MES SDS Running Buffer FAQs

Videos

How To Run the Bolt Pre-Cast Protein Gel

How to run the Bolt Bis-Tris Plus Pre-cast Protein gel on the Bolt Mini-Gel Tank. Do you run pre-cast protein gels? Western blotting? If you do, you should check out the New Bolt Pre-Cast Protein Gels and Bolt Electrophoresis Tank.

Video Player is loading.

Current Time 0:00

Duration 0:00

Loaded: 0%

Stream Type LIVE

Remaining Time 0:00