Die durch Fluoreszenzmikroskopie beobachtete Kolokalisierung von fluoreszierenden Cholera-Toxin-B-Konjugaten und der grün-fluoreszierenden BODIPY™ FL C5-Gangliosid GM1 liefert einen direkten Hinweis aufWeitere Informationen
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Katalognummer
Menge
B13950
auch als B-13950 bezeichnet
25μ g
Katalognummer B13950
auch als B-13950 bezeichnet
Preis (EUR)
544,00
Each
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Menge:
25μ g
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Preis (EUR)
544,00
Each
Zum Warenkorb hinzufügen
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Die durch Fluoreszenzmikroskopie beobachtete Kolokalisierung von fluoreszierenden Cholera-Toxin-B-Konjugaten und der grün-fluoreszierenden BODIPY™ FL C5-Gangliosid GM1 liefert einen direkten Hinweis auf die Assoziation dieser Moleküle in Lipid-Rafts.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Chemischer Name oder MaterialSphingolipide
Empfohlene LagerungBei -5 bis -30 °C lagern und vor Licht schützen.
Physikalische FormFest
ProduktlinieBODIPY™
Menge25μ g
Unit SizeEach
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Abbildungen
A J774 mouse macrophage cell stained with BODIPY® FL ganglioside GM1 (B13950) and Alexa Fluor® 555 dye–labeled cholera toxin subunit B.
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Zitierungen und Referenzen
Abstract
Manipulating FRET with polymeric vesicles: development of a "mix-and-detect" type fluorescence sensor for bacterial toxin.
Authors:Ma G, Cheng Q
Journal:Langmuir
PubMed ID:16863214
'A simple "mix-and-detect" type of fluorescence sensor for cholera toxin (CT) is reported. The sensor consists of a BODIPY lipid dye and polydiacetylene (PDA) vesicles and utilizes the lipid insertion and FRET mechanism to offer a direct and fluorescence "turn-on" detection of the analyte. BODIPY conjugated GM1, dissolved in a ... More
Ganglioside partitioning and aggregation in phase-separated monolayers characterized by bodipy GM1 monomer/dimer emission.
Authors:Coban O, Burger M, Laliberte M, Ianoul A, Johnston LJ,
Journal:Langmuir
PubMed ID:17477552
'The distribution of Bodipy GM1 in monolayers of binary and ternary lipid mixtures with coexisting fluid and ordered phases has been examined using a combination of atomic force microscopy and near-field scanning optical microscopy. Monolayers deposited at high (30 mN/m) and low (5 or 10 mN/m) surface pressures were examined ... More
T cell receptor can be recruited to a subset of plasma membrane rafts, independently of cell signaling and attendantly to raft clustering.
Authors:Giurisato E, McIntosh DP, Tassi M, Gamberucci A, Benedetti A
Journal:J Biol Chem
PubMed ID:12499387
'The constitutive/inducible association of the T cell receptor (TCR) with isolated detergent-resistant, lipid raft-derived membranes has been studied in Jurkat T lymphocytes. Membranes resistant to 1% Triton X-100 contained virtually no CD3epsilon, part of the TCR complex, irrespective of cell stimulation. On the other hand, membranes resistant either to a ... More
Correlated fluorescence-atomic force microscopy of membrane domains: structure of fluorescence probes determines lipid localization.
Authors:Shaw JE, Epand RF, Epand RM, Li Z, Bittman R, Yip CM
Journal:Biophys J
PubMed ID:16361347
'Coupling atomic force microscopy (AFM) with high-resolution fluorescence microscopy is an attractive means of identifying membrane domains by both physical topography and fluorescence. We have used this approach to study the ability of a suite of fluorescent molecules to probe domain structures in supported planar bilayers. These included BODIPY-labeled ganglioside, ... More
Applications of BODIPY-sphingolipid analogs to study lipid traffic and metabolism in cells.
Authors:Pagano RE, Watanabe R, Wheatley C, Dominguez M
Journal:Methods Enzymol
PubMed ID:11070900
17 total citations
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