NativePAGE™ Sample Prep Kit
NativePAGE™ Sample Prep Kit
Invitrogen™

NativePAGE™ Sample Prep Kit

The NativePAGE™ Sample Prep Kit includes sample preparation reagents for native gel electrophoresis. The kit includes two ready-to-use detergent solutions,Read more
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Catalog NumberQuantity
BN20081 kit
Catalog number BN2008
Price (USD)
324.00
Each
In stock
Add to cart
Quantity:
1 kit
Price (USD)
324.00
Each
Add to cart
The NativePAGE™ Sample Prep Kit includes sample preparation reagents for native gel electrophoresis. The kit includes two ready-to-use detergent solutions, 10% DDM (n-dodecyl-β-D-maltoside) and 5% Digitonin, which improve the solubility of hydrophobic and membrane proteins during sample preparation.

The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis, showing increased resolution and reduced streaking.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferSample Loading Buffers
Gel CompatibilityNativePAGE™ Gels
Product LineNativePAGE™
Product TypePAGE Sample Prep Kit
Quantity1 kit
Shipping ConditionWet Ice
Gel TypeNative-PAGE
Unit SizeEach
Contents & Storage
Contains:
• 1 ml of 10% DDM
• 1 ml of 5% Digitonin
• 10 ml of NativePAGE™ 4X Sample Buffer
• 0.5 ml NativePAGE™ 5% G-250 Sample Additive.

Store the kit at 2°C to 8°C.

Frequently asked questions (FAQs)

I would like to run a NativePAGE gel. Which of your protein standards should I use?

We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels?

We do not recommend using NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels. NuPAGE Invitrogen Bis-Tris gels are optimized for denaturing conditions and have an extremely low operating pH (pH 7.0), which makes it difficult for most proteins to migrate through them in a native state.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels or NuPAGE Tris-Acetate gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate or Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with NuPAGE Tris-Acetate gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate gels. When running Tris-Acetate gels under native conditions, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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