CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit
CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit
Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptoticRead more
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Catalog NumberQuantity
C10427Promo Image100 Assays
C10740Promo Image20 Assays
Catalog number C10427
Price (USD)
536.00
Each
In stock
Add to cart
Quantity:
100 Assays
Price (USD)
536.00
Each
Add to cart

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent, as well as SYTOX™ AADvanced™ Dead Cell Stain.

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers

CellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ∼511/533 nm. When used together with the SYTOX™ AADvanced™ Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/EmissionSYTOX AADvanced: 546/647
CellEvent Caspase 3/7 Green Detection Reagent: 511/533
Flow Cytometer Laser Lines488
For Use With (Application)Flow Cytometry
For Use With (Equipment)Attune™ Acoustic Focusing Cytometer, Flow Cytometer
Label TypeOther Label(s) or Dye(s)
Label or DyeCellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX AADvanced dead cell stain
Product LineCellEvent™
Product TypeCaspase Assay Kit
Quantity100 Assays
Shipping ConditionWet Ice
SolubilityDMSO (Dimethylsulfoxide)
Detection MethodFluorescence
FormatKit
Unit SizeEach
Contents & Storage
Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX AADvanced dead cell stain, and 1 vial of DMSO.

Store all vials in the kit upright, at or below -20deg;C, desiccated, and protected from light.

Frequently asked questions (FAQs)

I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use CellEvent Caspase-3/7 Green Detection Reagent with fixed samples?

No, it must be applied to live cell populations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I stain cells with nucleic acid stains?

Yes, you may use SYTOX AADvanced Dead Cell Stain that is included in the CellEvent Caspase-3/7 Green Ready Flow Reagent (Cat. No. R37167) and in the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Cat. No. C10427, C10740). You may also try using other nucleic acid stains in the red or far red channels for staining cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I multiplex with fluorescent immunolabeling?

Yes. You can apply surface labels or other reagents. We recommend applying the surface labels or other reagents first.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the CellEvent Caspase-3/7 Green Detection Reagent fixable?

The CellEvent Caspase-3/7 Green Detection Reagent is not covalently attached to any cellular component and may be lost upon fixation. For flow cytometry applications, after applying the reagent, you should examine without washing or fixation. For imaging applications, the CellEvent product signal may be retained after fixation in 3.7% formaldehdye for 15 min.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Fluorescence spectra

Fluorescence spectra

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