CoverWell perfusion chamber gaskets are designed to facilitate live-cell imaging and manipulation. These gaskets form watertight "press-to-seal" chambers with dual-access ports so reagents can be quickly added and removed. Available in single- or multi-well configurations, these gaskets allow multiple experiments to be performed on a single microscope slide or coverslip. The heat-resistant gaskets can be sterilized and used for direct culturing of cells and tissues.