CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry
See a minimum of 6 generations using the new CellTrace™ Far Red Proliferation Kit for Flow Cytometry.
CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry
Invitrogen™

CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry

CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generationsRead more
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Catalog NumberSufficient For
C34554Promo Image180 Reactions
C34570Promo Image20 Reactions
Catalog number C34554
Price (USD)
272.00
Each
Add to cart
Sufficient For:
180 Reactions
Price (USD)
272.00
Each
Add to cart

CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.

• Superior performance—bright, single-peak staining enables visualization of multiple generations
• Long-term signal stability—well-retained in cells for several days post stain
• Non-toxic—staining does not adversely effect cell health
• Simple, robust staining protocol

View a selection guide for all CellTrace™ Cell Proliferation Kits for flow cytometry.

Superior fluorescent staining
Successful proliferation analysis by dye dilution (see figure below) requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace™ CFSE dye enables the visualization of eight or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. Consistent, homogeneous staining results in very little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.

Long-term signal retention
Unlike stains that label the lipid membrane of cells, CellTrace™ CFSE dye easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye is designed to provide a consistent signal, even after several days in a cell culture environment.

Non-toxic
CellTrace™ CFSE dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells. Researchers have used CFSE SE labeling to show that transplantable hematopoietic cells proliferate in vitro in response to stimulation by a growth factor cocktail.

Simple, robust staining protocol
The CellTrace™ CFSE Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1 μL of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.

Consult user Manual for solubility instructions.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionCellTrace™ Cell Proliferation Kit, 180 rxns, CFSE, Lyophilized, Fluorescent Detection Method, 492/517nm Excitation/Emission Wavelength, CellTrace™ CFSE Label/Dye, –5 to –30°C Storage, Use with Flow Cytometer
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormLyophilized
FormatTube(s)
Quantity180 reactions kit
Reagent TypeCFSE & Related Compounds
Shipping ConditionRoom Temperature
SolubilityDMSO (Dimethylsulfoxide)
Sufficient For180 Reactions
Emission488
For Use With (Application)Proliferation Assay, Flow Cytometry
For Use With (Equipment)Flow Cytometer
Product LineCellTrace
Product TypeCell Proliferation Kit
Unit SizeEach
Contents & Storage
Contains 10 vials of CellTrace™ CFSE (lyophilized powder) and 1 vial of DMSO (500 μL). Store in freezer (-5°C to -30°C) and protect from light.

Frequently asked questions (FAQs)

I'm trying to label my cells in suspension and track them over time by labeling with CFDA SE, but after labeling them they will no longer adhere to a surface (whereas unlabeled cells adhere well). I've tried to label at 10 and 20 µM. What is causing this?

This dye will bind to proteins via primary amines. Too high of a concentration can lead to cell toxicity or unintended modification of cell function. The solution is to reduce the concentration and/or staining time, or to go with a non-protein-binding tracking reagent, such as Qtracker cell labeling reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the shelf life of CellTrace CFSE Cell Proliferation Kit (Cat. No. C34554, C34570)?

CellTrace CFSE Cell Proliferation Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (in freezer (-5 to -30 degrees C) and protected from light). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

For staining with CellTrace CFSE Cell Proliferation Kit, for flow cytometry (Cat. No. C34554 or C34570), up to how many cells can I have in 1 mL volume?

The cell concentration can be up to 1 x 10E7 cells/mL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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