Carboxy-DCFDA (5-(and-6)-Carboxy-2',7'-Dichlorofluorescein Diacetate), mixed isomers
Carboxy-DCFDA (5-(and-6)-Carboxy-2',7'-Dichlorofluorescein Diacetate), mixed isomers
Invitrogen™

Carboxy-DCFDA (5-(and-6)-Carboxy-2',7'-Dichlorofluorescein Diacetate), mixed isomers

Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterasesRead more
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Catalog NumberQuantity
C369100 mg
Catalog number C369
Price (EUR)
522,00
Each
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Quantity:
100 mg
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Price (EUR)
522,00
Each
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Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pKa of 4.8, this dye can be used as an acidic pH sensor.

To make a concentrated stock solution from 1–10 mM, dissolve in either high quality anhydrous DMSO, DMF, or 100% ethanol. Once prepared, use the stock solutions within a short time period.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypepH Indicator
Molecular Weight (g/mol)529.29
Quantity100 mg
Shipping ConditionRoom Temperature
For Use With (Application)Polar Tracer, pH Indicator
For Use With (Equipment)Fluorescence Microscope
Product TypeCarboxy-DCFDA
Unit SizeEach
Contents & Storage
Store in freezer (-5°C to -30°C).
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Figures

Fluorescence spectra

Fluorescence spectra

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Lot #Certificate TypeDateCatalog Number(s)
3135788Certificate of AnalysisApr 09, 2025C369
2930829Certificate of AnalysisMay 24, 2024C369
2500553Certificate of AnalysisJul 28, 2022C369
2379483Certificate of AnalysisSep 14, 2021C369
2282131Certificate of AnalysisMar 17, 2021C369
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Citations & References (48)

Citations & References
Abstract
Mitochondrial production of reactive oxygen species mediate dicumarol-induced cytotoxicity in cancer cells.
Authors:Du J, Daniels DH, Asbury C, Venkataraman S, Liu J, Spitz DR, Oberley LW, Cullen JJ
Journal:J Biol Chem
PubMed ID:17040906
'Dicumarol is a naturally occurring anticoagulant derived from coumarin that induces cytotoxicity and oxidative stress in human pancreatic cancer cells (Cullen, J. J., Hinkhouse, M. M., Grady, M., Gaut, A. W., Liu, J., Zhang, Y., Weydert, C. J. D., Domann, F. E., and Oberley, L. W. (2003) Cancer Res. 63, ... More
Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow.
Authors:Zheng J, Lu Z, Kocabas F, Böttcher RT, Costell M, Kang X, Liu X, Deberardinis RJ, Wang Q, Chen GQ, Sadek H, Zhang CC,
Journal:
PubMed ID:24385538
'How stem cells interact with the microenvironment to regulate their cell fates and metabolism is largely unknown. Here we demonstrated that the deletion of the cytoskeleton-modulating protein profilin 1 (pfn1) in hematopoietic stem cell (HSCs) led to bone marrow failure, loss of quiescence, and mobilization and apoptosis of HSCs in ... More
KEAP1 is a redox sensitive target that arbitrates the opposing radiosensitive effects of parthenolide in normal and cancer cells.
Authors:Xu Y, Fang F, Miriyala S, Crooks PA, Oberley TD, Chaiswing L, Noel T, Holley AK, Zhao Y, Kiningham KK, Clair DK, Clair WH,
Journal:
PubMed ID:23674500
'Elevated oxidative stress is observed more frequently in cancer cells than in normal cells. It is therefore expected that additional exposure to a low level of reactive oxygen species (ROS) will push cancer cells toward death, whereas normal cells might maintain redox homeostasis through adaptive antioxidant responses. We previously showed ... More
G-protein ligands inhibit in vitro reactions of vacuole inheritance.
Authors:Haas A, Conradt B, Wickner W
Journal:J Cell Biol
PubMed ID:8027189
'During budding in Saccharomyces cerevisiae, maternal vacuole material is delivered into the growing daughter cell via tubular or vesicular structures. One of the late steps in vacuole inheritance is the fusion in the bud of vesicles derived from the maternal vacuole. This process has been reconstituted in vitro and requires ... More
A trace amount of the human pro-apoptotic factor Bax induces bacterial death accompanied by damage of DNA.
Authors:Asoh S, Nishimaki K, Nanbu-Wakao R, Ohta S
Journal:J Biol Chem
PubMed ID:9556634
'An amount of human pro-apoptotic Bax as low as 0.01% of total protein was sufficient to cause cell death in Escherichia coli. The bacterial cell death was examined using a viable bacteria-specific fluorescence indicator system and loss of colony formation ability. Co-expression of anti-apoptotic Bcl-xL showed a modest inhibitory effect ... More
48 total citations

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