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Detect, enumerate and differentiate the members of coliform group with Thermo Scientific™ Oxoid™ Eosin Methylene Blue Agar (Modified) Levine (Dehydrated). This versatile medium, modified by Levine1,2, is used for the differentiation of Escherichia coli and Enterobacteria aerogenes and also for rapid identification of Candida albicans. The medium is prepared to the formula specified by the APHA3,4,5,6 for the detection and differentiation of the coliform group of organisms7,8.
| Description | Eosin-Methylene Blue Agar (Levine) |
| Form | Powder |
| Product Type | Agar |
| Quantity | 500 g |
| Yield | For 13.3L medium |
| Unit Size | Each |
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| CM0069B | Each | 500 g | 申請報價 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Use Eosin Methylene Blue Agar (Modified) Levine for isolation and differentiation of Enterobacterioceae.
- Easy-to-read: due to Eosin–Y and methylene blue indicators Escherichia coli (purple colonies with green metallic sheen) are easy to differentiate from Enterobacteria aerogenes (purple mucoid colonies).
- Recommended for the detection, enumeration, and differentiation of members of the coliform group by American Public Health Association.
Weld9,10 proposed the use of Eosin methylene blue agar (Modified) Levine, with added chlortetracycline hydrochloride for the rapid identification of Candida albicans in clinical materials. A positive identification of Candida albicans could be made after 24 to 48 hours incubation at 37°C in 10% carbon dioxide from feces, oral and vaginal secretions, and nail or skin scrapings.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
For Laboratory Use Only
General References:
- Levine M. (1918) J. Infect. Dis. 23. 43-47.
- Levine M. (1921) Bacteria Fermenting Lactose and the Significance in Water Analysis Bull. 62. Iowa State College Engr. Exp. Station.
- American Public Health Association (1980) Standard Methods for the Examination of Water and Wastewater. 15th Edn. APHA Inc. Washington DC.
- American Public Health Association (1978) Standard Methods for the Examination of Dairy Products. 14th Edn. APHA Inc. Washington DC.
- American Public Health Association (1992) Compendium of Methods for the Microbiological Examination of Foods 3rd Edn. APHA Inc. Washington DC.
- American Public Health Association (1970) Diagnostic Procedures. 5th Edn. APHA Inc. Washington DC.
- American Society for Microbiology (1974) Manual of Clinical Microbiology 2nd Edn. ASM Washington DC.
- Windle Taylor E. (1958) The Examination of Waters and Water Supplies 7th Ed., Churchill Ltd., London.
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