Utilize motility enrichment on Modified Semi-solid Rappaport Vassiliadis Agar (ISO) for the isolation of Salmonella species from food and environmental samples associated with the primary stage of animal production (such as dust).

• Simple and sensitive method for the isolation of salmonellae from food and environmental samples.
• Incorporated into ISO 6579:2002+A1:2007 Annex D¹, for the isolation of salmonellae from animal feces (such as poultry, pigs, and cattle).
• Gram-positive flora is inhibited by novobiocin, low pH, high magnesium chloride concentration, and malachite green.
• Isolation is based on the ability of salmonellae to migrate through the selective medium ahead of competing Gram-negative, motile organisms, thus producing opaque halos of growth.
• Presumptive identification is achieved by subculture onto XLD Agar and a second Salmonella agar of choice.

Modified Semi-solid Rappaport Vassiliadis Agar (ISO) is based on the formulation described by De Smedt et al., which has been shown to detect more Salmonella positive samples than traditional enrichment procedures. ²

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