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Invitrogen™
Número do catálogo: CS10203
Invitrogen™
Número do catálogo: CS10203
| Número do catálogo | Tamanho da unidade | Preço (BRL) | |
|---|---|---|---|
| CS10203 | 50 preps | Entre em contato › |
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Recomendações
Sample size 1 ml S. cerevisiae culture; Typical yield up to 25 ng
• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance
The ChargeSwitch™ Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 ml of Saccharomyces cerevisiae grown in selective minimal media. This kit typically yields up to 25 ng of plasmid DNA. Data generated from transformation and PCR experiments using ChargeSwitch™ purified DNA shows improved performance versus DNA purified by other methods (Figure 1). This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance
The ChargeSwitch™ Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 ml of Saccharomyces cerevisiae grown in selective minimal media. This kit typically yields up to 25 ng of plasmid DNA. Data generated from transformation and PCR experiments using ChargeSwitch™ purified DNA shows improved performance versus DNA purified by other methods (Figure 1). This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
Especificações
Format
Kit
Isolation Technology
ChargeSwitch Chemistry
Sample Type
Yeast
Scale
Mini
Final Product Type
Plasmid DNA
For Use With (Application)
PCR, Cloning, Southern Blotting, Sequencing, Transformation
Green Features
Less hazardous, Fewer resources used and less waste
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
50 Preps
Prep Scale
< 100 μg (Small-Scale) Plasmid DNA
Product Line
ChargeSwitch™
Product Type
Plasmid Yeast Mini Kit
Quantity
50 Preps
Shipping Condition
Room Temperature
Target
Plasmid DNA
Conteúdo e armazenamento
All components of the ChargeSwitch™ Plasmid Yeast Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store Precipitation Buffer (N5) at 4°C to improve performance. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.
Components:
• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch™ Resuspension Buffer (R4)
• ChargeSwitch™ Lysis Buffer (L9)
• ChargeSwitch™ Precipitation Buffer (N5)
• ChargeSwitch™ Wash Buffer (W11)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
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