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DH5α Competent Cells for Subcloning
Thermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Read more
Catalog number EC0111
Price (USD)/ 4 x 500 µL
59.65
Online exclusive
70.75 Save 11.10 (16%)
-
Price (USD)/ 4 x 500 µL
59.65
Online exclusive
70.75 Save 11.10 (16%)
DH5α Competent Cells for Subcloning
Catalog numberEC0111
Price (USD)/ 4 x 500 µL
59.65
Online exclusive
70.75 Save 11.10 (16%)
-
Thermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.
• Transformation efficiency: >1x106 cfu/μg pUC19 DNA
• Suitable for routine cloning applications
• Convenient economical packaging
• Contain genetic markers that allow for blue/white colony screening
Applications
DH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications:
• DNA cloning following PCR or restriction digestion reactions
• Subcloning (transferring DNA from a parent vector to a destination vector)
• Plasmid isolation
Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
• Transformation efficiency: >1x106 cfu/μg pUC19 DNA
• Suitable for routine cloning applications
• Convenient economical packaging
• Contain genetic markers that allow for blue/white colony screening
Applications
DH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications:
• DNA cloning following PCR or restriction digestion reactions
• Subcloning (transferring DNA from a parent vector to a destination vector)
• Plasmid isolation
Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeCompetent Cell
Contains F' EpisomeLacks F' Episome
Efficiency1x10^6 cfu/μg
Cell TypeChemically Competent
Cloning Methylated DNANo
Bacterial or Yeast StrainDH5α
Blue/White ScreeningYes
High-throughput CompatibilityNot High-throughput Compatible
Propagating ccdB VectorsNot for ccdB vector propagation
Shipping ConditionDry Ice
SpeciesE. coli
FormatTube
Product LineThermo Scientific™
For Use With (Application)Cloning
Quantity4 x 500 μL
Unit Size4 x 500 µL
Contents & Storage
• 4 x DH5α Competent Cells for Subcloning (500 μL)
• pUC19 DNA (50 μL) (100 pg/μL)
Store at -80°C.
• pUC19 DNA (50 μL) (100 pg/μL)
Store at -80°C.