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Exonuclease III (200 U/μL)
Exonuclease III (200 U/μL)
Thermo Scientific™

Exonuclease III (200 U/μL)

Thermo Scientific Exonuclease III (ExoIII) exhibits four catalytic activities. The 3'→5' exodeoxyribonuclease activity of ExoIII is specific for double-stranded DNA.Read more
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Catalog NumberQuantity
EN01914,000 units
Catalog number EN0191
Price (USD)
79.65
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89.00
Save 9.35 (11%)
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Quantity:
4,000 units
Request bulk or custom format
Price (USD)
79.65
Online Exclusive
89.00
Save 9.35 (11%)
Each
Add to cart
Thermo Scientific Exonuclease III (ExoIII) exhibits four catalytic activities. The 3'→5' exodeoxyribonuclease activity of ExoIII is specific for double-stranded DNA. ExoIII degrades dsDNA from blunt ends, 5'-overhangs or nicks, releases 5'-mononucleotides from the 3'-ends of DNA strands and produces stretches of single-stranded DNA. It is not active on 3'-overhang ends of DNA that are at least four-bases long and do not carry a 3'-terminal C-residue on single-stranded DNA, or on phosphorothioate-linked nucleotides.

ExoIII 3'-phosphatase activity removes the 3'-terminal phosphate, generating a 3'-OH group. ExoIII Rnase H activity exonucleolytically degrades the RNA strand in RNA-DNA hybrids. ExoIII apurinic/apyrimidinic-endonuclease activity cleaves phosphodiester bonds at apurinic or apyrimidinic sites to produce 5'-termini that are base-free deoxyribose 5'-phosphate residues.

Highlights

• Active in restriction enzyme buffers

Applications

• Creation of unidirectional deletions in DNA fragments in conjunction with S1 Nuclease
• Generation of a single-stranded template for dideoxy-sequencing of DNA
• Site-directed mutagenesis
• Cloning of PCR products
• Preparation of strand-specific probes

Note

The rate of DNA digestion by ExoIII depends upon temperature, salt concentration, and the molar ratio of DNA to enzyme in the reaction mixture . Optimal reaction conditions should be determined experimentally.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible BufferEnzyme Buffer
Product TypeExonuclease III
Quantity4,000 units
Concentration200 U/μL
EnzymeExonuclease
Unit SizeEach

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