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Klenow Fragment (10 U/μL)
Klenow Fragment (10 U/μL)
Thermo Scientific™

Klenow Fragment (10 U/μL)

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonucleaseRead more
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Catalog NumberQuantityConcentration
EP0051300 units10 U/μL
EP00521,500 units10 U/μL
Catalog number EP0051
Price (USD)
129.65
Online Exclusive
147.00
Save 17.35 (12%)
Each
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Quantity:
300 units
Concentration:
10 U/μL
Request bulk or custom format
Price (USD)
129.65
Online Exclusive
147.00
Save 17.35 (12%)
Each
Add to cart
Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.

Highlights

• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
• Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications
• DNA blunting by fill-in 5'-overhangs
• Random-primed DNA labeling
• Labeling by fill-in 5'-overhangs of dsDNA
• DNA sequencing by the Sanger method
• Site-specific mutagenesis of DNA with synthetic oligonucleotides
• Second strand synthesis of cDNA
For Research Use Only. Not for use in diagnostic procedures.
Specifications
PolymeraseDNA Polymerase I
Product TypeKlenow Fragment
Quantity300 units
Concentration10 U/μL
Unit SizeEach

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