AloI (2 U/μL)
AloI (2 U/μL)
Thermo Scientific™

AloI (2 U/μL)

5' ↓N7G A A C N6T C C N12-13↓ 3'3' ↑N12-13C T T G N6A G G N7↑ 5'Thermo ScientificRead more
Have Questions?
Catalog NumberQuantity
ER1491100 units
Catalog number ER1491
Price (USD)
223.65
Online Exclusive
242.00
Save 18.35 (8%)
Each
-
Add to cart
Quantity:
100 units
Request bulk or custom format
Price (USD)
223.65
Online Exclusive
242.00
Save 18.35 (8%)
Each
Add to cart
Ask our AI about this Product
5'  N7G  A  A  C  N6T  C  C  N12-13 3'
3'  N12-13C  T  T  G  N6A  G  G  N7 5'

Thermo Scientific AloI restriction enzyme recognizes ^(7/12-13)GAAC(N)6TCC(12-13/7)^ sites and cuts best at 30°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: For methylation sensitivity, refer to product specifications. Incubation at 37°C results in 20% activity. AloI produces double-strand cuts on both sides from the interrupted recognition site. Its unique feature is a degenerate cleavage point on the 3 side of the recognition sequence (12 or 13 nt away). The presence of SAM in the reaction mixture results in incomplete cleavage with AloI. Greater than 10-fold overdigestion with AloI may result in star activity. AloI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible Buffer10X Buffer R
Product TypeRestriction Enzyme
Quantity100 units
Concentration2 U/μL
EnzymeAlo I
Methylation SensitivityCpG Methylation-Sensitive, Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Optimal Reaction Temperature30°C
Research CategoryTraditional Cloning
Sensitive to Heat InactivationYes
Type IIS RENo
Unit SizeEach
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link