DpnI (10 U/μL)
DpnI (10 U/μL)
DpnI (10 U/μL)
DpnI (10 U/μL)
Thermo Scientific™

DpnI (10 U/μL)

5'GAm6↓TC3'3'CT↑Am6G5'Thermo Scientific DpnI restriction enzyme recognizes Gm6A^TC sites and cuts best at 37°C in Tango buffer (isoschizomers: MalI). See ReactionRead more
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Catalog NumberQuantity
ER17051,000 units
ER1701500 units
ER17022,500 units
Catalog number ER1705
Price (TWD)/ Each
2,080.00
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Ends: 30-Jun-2025
3,050.00
Save 970.00 (32%)
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Quantity:
1,000 units
Request bulk or custom format
Price (TWD)/ Each
2,080.00
Online offer
Ends: 30-Jun-2025
3,050.00
Save 970.00 (32%)
Add to cart
5'GAm6TC3'
3'CTAm6G5'

Thermo Scientific DpnI restriction enzyme recognizes Gm6A^TC sites and cuts best at 37°C in Tango buffer (isoschizomers: MalI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: DpnI requires the presence of N6-methyladenine within the recognition sequence to cleave DNA. DNA purified from a dam+ strain will be a substrate for DpnI. DpnI will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam sites DpnI cleaves 60X more slowly. DpnI, Bsp143I, and MboI all recognize the same sequence, but have different methylation sensitivities and cleavage sites. Assayed using pBR322 DNA. For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible Buffer10X Buffer Tango
Product TypeRestriction Enzyme
Quantity1,000 units
Concentration10 U/μL
EnzymeDpn I
Methylation SensitivityDam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Optimal Reaction Temperature37°C
Research CategoryTraditional Cloning
Sensitive to Heat InactivationYes
Type IIS RENo
Unit SizeEach

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