Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant, fungi and bacteria.

The kit includes a complete set of optimized PCR reagents as well as detailed protocols for different sample types, making it an ideal choice for amplification of plant DNA. In addition, the kit includes control primers for positive control reactions.The Control Primers amplify a highly conserved region of chloroplast DNA and are universal for many plant species.

Highlights

• No need for time-consuming and expensive DNA purification steps
• Very little sample material required
• Two simple protocols for various applications
• Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb).

Applications

• Genotyping
• Transgene detection

The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq -based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.