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货号 | 数量 |
---|---|
F20017 | 100块板 |
F20016 | 10块板 |
F20015 | 2 板 |
FluxOR™ II 绿色钾离子通道检测试剂盒是高通量筛选(High Throughput Screening,以下简称 HTS)钾离子通道和转运体活性的下一代溶液。FluxOR II 绿色钾离子通道检测试剂盒利用了钾通道对铊离子的可透过性。其采用了改良的铊敏感荧光指示剂染料,可获得较大的信号窗口,显著提高检测的灵敏度。此外,检测步骤有“清洗”或“无清洗”两种形式。
特点包括:
•较高的检测灵敏度和较大的信号窗口有助于分析更大范围的钾通道,包括低表达或弱传导通道
• 与上一代产品相比,动态范围增加了 100%;与竞争产品相比,动态范围增加了 >40%
• 均相、无清洗的方案可减少孔间差异并提高检测重现性
• 针对高通量筛选应用进行优化
• 规格适用于低至高通量用户
与上一代检测相似,FluxOR II 绿色钾离子通道检测利用了钾离子通道对铊的可透过性。当刺激物打开钾通道时,细胞外的铊顺着浓度梯度流入细胞,通过预先加载到细胞中的专有的指示剂染料,来检测离子通道或转运体活性,在铊存在的情况下,细胞溶质荧光增强。FluxOR II 检测通过这种方式观察到的荧光是使得铊进入细胞的离子通道活性或转运过程的指示剂。该荧光信号可定量反映对铊具有可透过性的离子通道和转运体的活性,包括 hERG、Kir2.1 和其他药理学上重要的钾通道。对于 FluxOR II 检测试剂盒,开发了一种改良指示剂染料,可获得更大的信号窗口,提高检测灵敏度。
除了改良荧光指示剂染料外,FluxOR II 绿色钾离子通道检测试剂盒还提供了浓缩的铊溶液和必需的缓冲液,具有较大的靶标灵活性和操作便利性的均质形式,这已在稳定表达 hERG 的细胞中得到证实。检测步骤有“清洗”或“无清洗”两种形式。
The FluxOR Red Potassium Ion Channel Assay is recommended for cells that are expressing GFP or related fluorescent proteins or with cells that have high autofluorescence in the green emission channel.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The FluxOR II Green Potassium Ion Channel Assay can be used with common FITC filter settings and the FluxOR Red Potassium Ion Channel Assay can be used with standard TRITC filter settings. For cells that are expressing GFP or have high autofluorescence in the green emission range, FluxOR Red is an alternative. If cells are expressing dsRED, mCherry or other red-emitting fluorescent proteins or exhibit high autofluorescence in the red emission range, FluxOR II Green is an excellent option.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The FluxOR II assay utilizes an improved thallium-sensitive fluorogenic indicator dye that results in a larger signal window and a significant improvement in the sensitivity of the assay.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
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